摘要
以患丛枝病豫杂一号泡桐叶片、叶柄和茎段为外植体,建立了其体外植株再生系统.结果表明,其叶片、叶柄和幼嫩茎段在MS+NAA 0.3 mg.L-1+6-BA 12 mg.L-1,MS+NAA 0.3 mg.L-1+6-BA 8 mg.L-1和MS+NAA 0.3 mg.L-1+6-BA 12mg.L-1组合培养基上,最高芽诱导率可达47.78%,21.11%和42.22%.此外,不含NAA和IBA的1/2MS培养基可作为患病苗幼芽生根的最适培养基.研究结果可为泡桐的基因工程操作和阐明丛枝病发生机理奠定基础.
Establishment of plantlet regeneration system with leaves, petioles and nodal segments of Paulownia tomentosa×Paulownia fortunei with witches＇ broom was studied in this paper. The results indicated that the highest shoot induction rates of the leaves, petioles and nodal segments on the following combined media, MS ＋ NAA 0. 3 mg·L^-1＋ 6-BA 12mg·L^-1,MS＋ NAA0.3 mg·L^-1＋6-BA8 mg·L^-1 and MS＋ NAA0.3 mg·L^-1 ＋ 6-BA 12 mg·L^-1 were respectively 47.78% ,21.11% and 42.22% .1/2MS free NAA and IBA might be the optimal medium for shoot rooting induction. These results might lay solid foundation for the manipulation of gene engineering of P. tomentosa × P .fortunei with witches＇ broom.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2005年第3期254-258,共5页
Journal of Henan Agricultural University
基金
国家自然科学基金资助项目(30271082)
河南省高校创新人才基金资助项目(2001012)
关键词
泡桐
丛枝病
培养基
体外植株再生
P. tomentosa ×P.fortunei
witches＇ broom
N1edium
in vitro plantlet regeneration
