摘要
我们首先报道了ＲＡＰＤ－ＰＣＲ技术研究微小牛蜱ＤＮＡ的多态性。本文则用３个引物＂Ｐｚ＂（ＣＧＧＣＣＣＧＧＴＡ）、＂Ｐｗ＂（ＣＧＧＣＣＣＣＧＧＴ）和＂Ｐｙ＂（ＣＴＧＣＡＧＧＡＣＡ）对亚洲璃眼蜱基因组ＤＮＡ作ＲＡＰＤ－ＰＣＲ，均能扩增出多态性ＤＮＡ图谱；各引物扩增的ＤＮＡ片段的分子大小是：＂ｐｚ＂为１３８０、１２３０、９５５、９１２、８７１、７９４、７４１、６６８、６４６、５６２、５３７、４４２；＂ｐｗ＂为９５５、７２４、６６８、５６２、５３７、４５７、２７９；＂Ｐｙ＂为１０７２、９１２、８３２、７９４、６１２、５８２、５６２、５３７、４２２、３８５。ＲＡＰＤ－ＰＣＲ技术简便、快速，并能在ＤＮＡ水平上揭示其差异和进化关系，可发展成为理想的蜱类分类学技术之一。
We reported the study on polymorphism DNA of Boophilus microplus with RAPD-PCR technique in the first instance.Here,we amplified the genomic polymorphism DNA maps of Hyaloma asiaticum asiaticum with three primers-“Pz” (CGGCCCGGTA),“Pw”(CGGCCCCGGT) and“Py”(CTGGAGGACT).The molecular weights (bp) of the DNA bands for each map were:“Pz”: 1380,1230,955,912,871,794,741,668,646,562, 537,442; 'Pw':955,724,668,562,537,457,279; 'Py':1072,912,832,794,612,582,562,537,422,385. The RAPD-PCR for its simplicity, rapidness,and being able to reveal difference and evolutional relationships at the level of DNA,could be developed into an ideal taxonomic method for Acarology.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1996年第2期101-104,共4页
Chinese Journal of Vector Biology and Control
