摘要
[目的]探讨用紫外分光度法测保健食品中谷胱甘肽。[方法]2004年8月采用谷胱甘肽原料药作对照品,样品于碱性条件下超声波提取,在230nm处测吸光度。[结果]本法标准曲线相关系数r=0.9992;精密度良好,相对标准偏差<5%;加标回收率在98%～104%之间。[结论]该方法操作简单,灵敏度高,准确度好。
[Objective]To determine Glutathione in healthy food by UV Spectrophotometry. [Methods]The raw drug of Glutathione was used as reference substance. Sample in alkaline medium was extracted by ultrasonic. Absorption of Glutathione at 230nm was determined for Giutathonione assay. [Results]The correlation coefficient of standard curve as 0. 9992 ; relative standard deviation（RSD） as 〈5 %; recovery percent as 98%-104%.[Conclusion]The method was simply, sensi- tive and accurate.
出处
《预防医学论坛》
2005年第5期560-561,共2页
Preventive Medicine Tribune
