摘要
目的:观察γ-干扰素(IFN-γ)对血管内皮细胞(EC)的作用及其对肿瘤坏死因子-α(TNFα)表达的影响。方法:体外培养EC,MTT法检测IFN-γ(0、20、40、80μg/L)对EC生长的直接影响,半定量逆转录-多聚酶链反应检测不同浓度的IFN-γ对EC凋亡相关基因TNFαmRNA的表达的影响。结果:IFN-γ可抑制体外培养的EC生长(P<0.01),且具有剂量依赖;IFN-γ能明显上调EC的TNFα表达。结论:IFN-γ可能通过上调EC中TNFα的表达而诱导EC凋亡。
Objective：To explore the role of interferon-γ（IFN-γ） in inducing the apoptosis of vascular endothelial cells（EC） in vitro and its influence on the expression of TNFα. Methods：The EC cells were cultured in vitro with the condition media containing varied concentration of IFN-γ（0,20,40,80μg/L） for 24 hours,and then the total RNA was extracted to analyze the mRNA level of TNFα in EC by RT-PCR. The influence of IFN-γ in inducing the apoptosis on EC was analyzed by MTT method. Results： IFN-γ could induce the apoptosis of EC in a dose-dew.ndent manner and it could upregulate the expression of TNFα mRNA level in EC. Conclusions： IFN-γ can induce the apoptosis of EC in vitro by up-regulating the expression of TNFα.
出处
《蚌埠医学院学报》
CAS
2005年第5期388-389,共2页
Journal of Bengbu Medical College
基金
安徽省科技厅重点科研项目(0401010)
关键词
动脉粥样硬化
干扰素类
血管内皮细胞
凋亡
artherosclercsis
interferons
vascular endothelial cell
apoptosis
