摘要
目的探讨“乌头反贝母”的科学内涵。方法建立血浆固相萃取富集方法及合适的HPLC条件流动相由甲醇水氯仿三乙胺(70∶30∶2∶0.2)组成,流速1.0mL·min-1,检测波长235nm,柱温40℃;对家兔静脉给药动物血浆中新乌头碱、乌头碱、次乌头碱进行测定。结果新乌头碱、乌头碱与浙贝母总生物碱配伍应用前后,其药动学参数变化二种乌头生物碱与浙贝母总生物碱配伍应用后,其消除半衰期均比单用延长(P<0.05),血浆清除率均比单用减慢(P<0.05),AUC均比单用增大(P<0.05);次乌头碱与浙贝母总生物碱配伍应用后,其药动学参数与单用次乌头碱比,主要参数差异不显著(P>0.05)。结论乌头生物碱与浙贝母总生物碱配伍应用后在动物体内的滞留时间延长和效用时间延长。从药动学角度初步验证了“乌头反贝母”理论的科学性。
Objective： To probe the counteractiv emechanism of “aconite counteracts fritillaria”. Methods： We used Solid phase extraction（SPF）to extract and enrich the components contained in the plasma. Chromatographic condition： Methyl alcohol-water-chloroform-triethylamine（70：30 ： 2 ： 0.2） was used as the mobile phase, the flow rate was 1.0mL·min^-1 with detection wavelength at 235nm and column temperature at 40℃ ; detecting the contents of mesaconitine, aconitine and hypaconitine. Result： By using singly or mixed with thunberg fritillary bulb omni-alkaloids, there were obvious differences between these pharmacokinetic parameters of mesaconitine and aconitine： the elimination half-lifes and AUCs of two aconite alkaloids after used with thuberg fritillary bulb omni-alkaloids were longer or higher than those before matching, and the plasma clearances of two aconite alkaloids after used with thunberg fritillary bulb omni-alkaloids were lower than these before matching（P〈0.05）. There were unsignificanted differences of hypaconitine used singly and used mixed with thunberg fritillary bulb omni-alkaloids （P〉0.05）. Conclusion： Retention time and affection time of aconite alkaloids after used with thunberg fritillary bulb omni-alkaloids were longer than these before matching. The statement of “aconite counteracts fritillaria” is preliminarily validated by our pharmacokinetic studies.
出处
《中国实验方剂学杂志》
CAS
2005年第5期24-27,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点基础研究发展规划项目(G1999055409)
