摘要
目的分析自身免疫性溶血性贫血(A IHA)患者外周血T细胞亚群极化状态,探讨A IHA的免疫学发病机制。方法收集A IHA患者及健康者外周抗凝静脉血,分离纯化淋巴细胞。F ITC标记的抗CD 3单抗,Cy5标记的抗CD 4、CD 8单抗,PE标记的CRTH 2单抗,以CD 3/CD 4、CD 3/CD 8设门作双色流式细胞术,分析A IHA患者T细胞亚群极化状态。结果与正常对照相比,A IHA患者外周血CD 4+细胞百分率显著升高(P<0.01),CD 8+细胞百分率变化不显著(P>0.05),CD 4+/CD 8+比例显著升高(P<0.05);CD 3+CD 4+CRTH 2-T细胞百分率、CD 4+CRTH 2-T/CD 4+CRTH 2-T和CD 8+CRTH 2-T/CD 8+CRTH 2+T比例均显著升高(P<0.01),而CD 3+CD 4+CRTH 2+T、CD 3+CD 8+CRTH 2-T及CD 3+CD 8+CRTH 2+T细胞百分率的变化不显著(P>0.05)。结论A IHA患者外周血存在细胞免疫功能失调,T细胞亚群极化状态发生改变,呈明显的T h1/T c1型细胞优势;T h1/T c1型细胞介导的细胞免疫可能与A IHA的免疫学发病机制有关。
Objective To investigate the change of Thl-like and Th2-1ike cells balance in AIHA patients. Methods The ratios of Th/Tc ,Th1/Th2 and Tcl/Tc2 in peripheral blood T cells were analyzed by immunofluorescence staining and bicolor flow cytometry （FCM） in vitro. Results Compared with the ratios of Th1/Th2 （48. 76%± 6. 17%） and Tc1/Tc2（18.90%±4.12%） in healthy people,the ratios of Th1/Th2（56.21%±5.95%） and Tc1/Tc2（23.09%± 3.31%） in AIHA patients increased obviously. FCM analysis showed that average percentages of Th,Th1 ,Th2,Tc ,Tc1 and Tc2 are 22.31%±6.51%,21.92%±6.42%,0.39%±0.14%,31.12%±6.15%,30.95%±5.45% and 1.34%±0.84% in AIHA patients versus 39.24%±5.82%, 39.01%±5.47%,0.80%±0.16%, 30.25%±5.63%,28.72%± 5.20 % and 1.52 % ±0.68% in healthy people. The average percentages of Th ,Th1 and Th2 decreased obviously. While the average percentages of Tc ,Tc1 and Tc2 did not changed. Conclusion The ratios of Th1/Th2 and Tc1/Tc2 in peripheral blood T cells were increased obviously in AIHA patients. It suggested that cellular immunity in AIHA patients is shifted to Th1 type immunity.
出处
《临床输血与检验》
CAS
2005年第4期244-247,共4页
Journal of Clinical Transfusion and Laboratory Medicine
