摘要
以PSA分散固相萃取净化,液相色谱柱分离,直接荧光法测定灭定威,研究建立了测定蔬菜、水果中灭定威的分析方法.在仪器最佳测定条件下,灭定威的检出限为2.98pg,在所测定的含量范围内灭定威的含量与峰面积之间的相关系数为1.000,保留时间的相对标准偏差为0.64%;PSA分散固相萃取法与氨基柱固相萃取法相比,操作简单,基体干扰少,结果准确可靠,重复性好.实际样品中灭定威的平均回收率在89%～112%之间,测得量的相对标准偏差在1.6%～5.6%之间,测定结果令人满意.
Pirimicarb in vegetables and fruits was determined with PSA dispersive solid- phase extraction cleanup and HPLC fluorescence detection. Under the optimized instrumental conditions, the detection limit of pirimicarb was 2.98 pg. A good linear relationship was obtained between the concentration and peak area of pirimicarb within the preset concentration range, with the correlation coefficient being 1. 000. The relative standard deviation of retention times was 0.64% . Compared to NH2 colunm solid-phase extraction, PSA dispersive solid-phase extraction was easier to operate with fewer matrix interferences, and achieved better results. The average recoveries of pirimicarb spiked in vegetable and fruit ranged from 89% to 112%, with the relative standard deviations ranging from 1.6% to 5.6% .
出处
《分析测试学报》
CAS
CSCD
北大核心
2005年第5期75-77,81,共4页
Journal of Instrumental Analysis
关键词
PSA
分散固相萃取
液卡甘色谱
荧光法
灭定威
蔬菜
水果
PSA
Dispersive solid- phase extraction
HPLC
Fluorescence detection
Pirimicarb
Vegeta bles
Fruits
