摘要
目的:建立固相萃取-高效液相色谱法测定氯雷他定血药浓度的方法.方法:采用ODS-C18小柱处理样品,Zorbax SB-C18柱(4.6 mm× 250 mm,5μm),流动相:50mmol·L-1磷酸二氢铵缓冲液(磷酸调至pH 4.0)-乙腈(62:38),流速:1.2 mL·min-1,检测波长:275 nm.结果:线性范围1～100 μg·L-1(r=0.999 6),回收率在82.63%～92.41%,日内、日间RSD＜10%,最低定量浓度为1μg·L-1.结论:该法简便、快速、准确,适用于氯雷他定药动学和生物等效性研究.
OBJECTIVE To establish a method for the determination of Loratadine in human plasma using a solid-phase extraction and reversed-phase HPLC. METHODS The sample was treated with ODS-18 solid-phase extraction column, Zorbax SB-18 column （4. 6 min×250 mm,5μm）was used,50 mmol·L^-1 ammoninium dihydrogen phosphate solution（adjust pH to 4. 0 with H3PO4 ）-acetonitrile（62：38）were served as mobile phase with 1.2 mL·min^-1 flow rate. The detection wavelength was 275 nm. RESULTS Loratadine concentration presented a good linear range of 1 - 100μg·L^-1 （r= 0. 999 6）, the recovery was between 82. 63% - 92. 41%, the relative standard deviations, of within-day and between-day were less than 10%. The limit of quantitation was 1μg· L^-1. CONCLUSION This method is rapid, simple and accurate for the determination of Loratadine levers in human plasma. It is suitable for the pharmacokinetics and bioavailability study of loratadine.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第9期830-831,共2页
Chinese Journal of Hospital Pharmacy
