摘要
目的研制丹参酮脂质传递体,进行体外小鼠经皮渗透研究。方法采用薄膜超声法制备脂质传递体。以改进的Franz扩散池进行体外经皮渗透实验,比较不同给药形式和给药体积对丹参酮经皮渗透的影响。结果脂质传递体粒径79.7nm,包封率达81.2。脂质传递体12h累积透过量为(1024±104.0)ng/cm2,皮肤滞留量为(345.2±58.0)ng/cm2;普通脂质体12h内接收介质中未检测到药物,皮肤滞留量为(625.3±124.5)ng/cm2,水醇混悬液仅在给药8h后才检测到少量药物,皮肤滞留量为(765.0±60.00)ng/cm2,随脂质传递体给药体积增大(0.1,0.2,0.5mL),12h皮肤累积透过量提高(依次为676.4±63.69、1024±104.0、1410±77.20ng/cm2),透皮速率增加(分别为63.78±7.79、97.27±9.14、140.0±5.50ng(cm2·h)),皮肤滞留量增加(依次为64.70±7.50、345.2±58.0、628.3±98.11ng/cm2)。结论脂质传递体可以促进丹参酮经皮转运。
AIM ： To prepare ethosomes and study on transdermal resorption of tanshinone ethosomes in vitro. METHODS： Tanshinone ethosomes were prepared by thin-film ultrasonic method. The different dosage regimens and volume on the transdermal resorption of tanshinone were evaluated through excised mice skin in vitro using modified Franz-diffustion cells. RESULTS ： The mean particle diameter of ethosomes was 79.7 nm, and the encapsulation efficiency was about 81.2%. Cumulative penetration amount of tanshinone from ethosomes was 1024 ± 104.0 ng/cm^2 and skin residual amount was 345.2 ± 58.0 ng/cm^2. Cumulative penetration amount increased with ethosomes volume. Liposomes failed to promote the penetration of tanshinone, and its skin residual amount was 625.3 ±124.5 ng/cm^2, water-ethanolic suspension also could not promote the penetration of tanshinone, and skin residual amount was 765.0 ± 60.00 ng/cm^2. CONCLUSION：Ethosomes can improve significantly the transdermal delivery of tanshinone.
出处
《中成药》
CAS
CSCD
北大核心
2005年第9期1000-1003,共4页
Chinese Traditional Patent Medicine
基金
江苏省科委高新技术招标项目(BG2002023)
关键词
丹参酮
丹参酮ⅡA
脂质传递体
透皮吸收
体外
tanshinone
tanshinone Ⅱ A
ethosomes
transdermal resorption
in vitro
