摘要
将中华绒螯蟹Eriocheir sinensis不同发育期的胚胎从腹肢基部剪下后在室温下孵化,结果表明,处于卵裂期、囊胚期和原肠期的胚胎离体孵化的孵化率(42.16%～54.90%)无显著差异(P＞0.05);从原肠期开始,离体孵化的孵化率随着离体时间的缩短呈升高的趋势,即眼点期(68.95%±5.89%)和心跳期(77.47%±5.45%)极显著高于(P＜0.01)前3个时期,对外界环境的适应性也增强,这5个时期离体胚胎孵出的溞状幼体的变态率差异不显著(P＞0.05).试验证明,剪掉抱卵腹肢进行胚胎离体孵化,不但促进了亲蟹卵巢的快速发育,而且还降低了投喂、调节水质等费用.
Cleavage, blastula and gastrula were separated from matured Chinese mitten crab and incubated in vitro. The results showed that the hatching rate, varying from 42.16% to 54.90%, was no significant difference （P〉0.05 ）, even though there was an in increase in hatching rate with the embryonic development （eyed stage, 68.95%±5.89%; heart beating stage, 77.47%±5.45%）. The metamorphosis of the larvae from the stages above was the same, indicating that this is feasible for efficient commercial larva production.
出处
《大连水产学院学报》
CSCD
北大核心
2005年第3期192-197,共6页
Journal of Dalian Fisheries University
基金
教育部"新世纪高等教育教学改革工程"资助项目(1292B0611)
关键词
中华绒螯蟹
受精卵
离体
幼体
发育期
孵化
Eriocheir sinensis
fertilized egg
in vitro
larva
developmental stage
incubation
