摘要
目的探讨苦荞麦籽粒提取物(FTGE)对肝脏的保护作用。方法FTGE对四氯化碳(CC l4)致急性肝损伤的作用:小白鼠60只,随机分为6组,每组10只。正常对照组、CC l4模型组灌胃给予等量纯化水,阳性对照组灌胃给予联苯双酯200 mg.kg-1.d-1,低剂量实验组、中剂量实验组、高剂量实验组分别灌胃给予FTGE 20,40和60g.kg-1.d-1,各组均连续给药7 d。7 d后,除正常对照组外,其他各组小鼠均腹腔注射0.1%CC l4花生油溶液10mL.kg-1。禁食16 h,摘眼球取血,分离血清,测试血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)值。FTGE对D-半乳糖胺(D-Galn)致急性肝损伤的作用:取60只小鼠,雌雄各半,分为6组,每组10只。正常对照组、D-Galn模型组灌胃给予0.9%氯化钠溶液20 mL.kg-1.d-1,阳性对照组灌胃给予联苯双酯15 mg.kg-1.d-1,低剂量实验组、中剂量实验组、高剂量实验组分别灌胃给予FTGE 20,40和60 g.kg-1,各组连续灌胃给药12 d,于末次给药后1 h,除正常对照组外,其他各组均腹腔注射10%D-Galn 800 mg.k-g1,小鼠于腹腔注射D-Galn后禁食过夜,待16 h后断头处死动物。结果FTGE对CC l4和D-Galn导致的急性肝损伤小鼠有非常显著的降酶作用(P<0.01),且剂量越大,降酶作用越强。结论苦荞麦籽粒提取物对化学性肝损伤小鼠有明显的保护作用。
Objective To probe into the protective effect of the ethanol extract of the seed of Fagopyrum tartaricum （FTGE） on the liver acutely injured by chemicals in mice. Methods 120 mice of the Kunming strain served as the subjects of the experiment. For the study of the effect of FTGE on the liver injured by CCl4, 60 mice were randomly divided into 6 equal groups. Mice of the low dose, medium dose and high dose groups were given each by gastragavage 20, 40 and 60g·kg^-1·d^-1 of FTGE for 7 consecutive days, respectively. Mice of the normal control group and CCl4 model group were given each equivalent amount of purified water by the same route for 7 days. Mice of the positive control group were given each 200 mg·kg^-1·d^-1 of bifendate by gastrogavage as well for 7 days. Except for the mice of the normal control group, animals of all the other 5 groups were given each 7 days later an intraperitoncal injection of 10 mg·kg^-1 of 0.1% CCl4 peanut oil solution. After a 16 h. fasting, blood samples were collected for the determination of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） while liver tissue was taken for pathological examination. For the study of the effect of FIGE on the liver injured by galactosamine（D-Gain）, 60 mice were divided into 6 equal groups. Mice of the low, medium and high dose groups were given each by gastrogavage 20, 40 and 60g·kg^-1·d^-1 of FIGE for 12 consecutive days. Mice of the normal control group and D-Gain groups were given each 20 mL·kg^-1·d^-1 of 0.9% sodium chloride solution by the same route for 12 days. Mice of the positive control group were given each 15 mg·kg^-1·d^-1 of bifendate by gastrogavage as well for 12 days. Except for mice of the normal control group, rodents of all the other 5 groups were given each 1 h. after the last gastrogavage an intraperitoneal injection of 800 mg·kg^-1 of 10% D-Gain. After a 16 h fast, the mice of all 6 groups were sacrificed and blood samples were collected for the determination of serum ALT and AST while liver tissue was taken for pathological examination. Results FTGE was shown to lower the activities of serum ALT and AST very strikingly in a dose dependent manner in mice with acute liver injury induced by chemical hepatotoxins CCl4 and D-Galn（P〈0.01）. A dramatic alleviation of the histopathological changes of the liver was also demonstrated in mice pretreated with FTGE before the induction of liver injury as compared with those of mice of the CCl4 and D- Gain model groups without FTGE pretreatment（P〈0.01）. Conclusion FTGE was shown to exert a remarkable protective effect on the murine liver acutely injured by CCl4 and D-Gain.
出处
《医药导报》
CAS
2005年第10期880-882,共3页
Herald of Medicine
关键词
苦荞麦籽粒提取物
四氯化碳
D-半乳糖胺
肝损伤
Extract of the seed of Fagopyrum tartaricum（FTGE ）
Carbon tetrachloride
D-galactosamine
Liver injury
