摘要
通过单因素考察及正交试验优化了用高压脉冲电场提取中国林蛙多糖的试验条件,并与碱提取法、酶提取法以及复合酶提取法进行了比较。结果显示用0.5%KOH提取液,在电场强度为20kv/cm和脉冲数为6μs的条件下用高压脉冲电场提取林蛙多糖的提取率最大为55.59%。比较高压脉冲电场提取法与碱法、酶法以及复合酶法在林蛙多糖提取率、总糖含量方面的差异,高压脉冲电场提取的林蛙多糖提取率和总糖含量均高于其他三种方法,其提取率是复合酶法的1.77倍,总糖含量高于复合酶法6.34%,且提取物中杂质少。因此,高压脉冲电场提取多糖是一种很有希望的多糖提取新方法。
In order to gain the optimization extract of polysaccharide of Rana chensinensis David by pulsed electric field （PEF）, we have inspected single factor influence and then optimized them by orthogonal experiment. The result was showed that the largest extraction ratio is 55.59% by PEF on the conditions of 0.5% KOH and 20kv/cm electric field intensity and 6 μs pulse number. Compared it with the alkali extract method and enzyme extract method and compound extract method, the extract ratio and polysaccharide content of PEF method are higher than the other three methods. The PEF extract ratio is 1.77 time of compound enzyme method and total sugar contents is more 6.34% than compound enzyme method and the impurity of extraction material is less. So PEF method is a novel and promising method to extract polysaccharide.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第9期337-339,共3页
Food Science
关键词
高压脉冲电场
林蛙多糖
正交试验
pulsed electric field（PEF）
polysaccharide of Rana chensinensis David
orthogonal experiment
