摘要
目的探讨载脂蛋白J(ApoJ)外显子3、4、7、8基因多态性与2型糖尿病(T2DM)的关系.方法 2002-11～2003-06对贵阳医学院附属医院,利用聚合酶链式反应-变性梯度凝胶电泳技术(PCR-DGGE)对61例T2DM患者、60例正常对照者的ApoJ外显子3、4、7、8基因进行基因筛查,并对异常条带进行测序分析.结果我国ApoJ基因外显子7缺失与插入(D VS I)基因型在T2DM与正常对照组间分布差异显著(P<0.01),ApoJ外显子3、4、8差异无显著性(P>0.05).结论我国2型糖尿病患者ApoJ外显子7基因插入与缺失多态性可能与2型糖尿病发病有关联,是2型糖尿病的危险因素之一.
Objective To investigate the association between apolipoprotein J （ApoJ） gene polymorphism and type 2 diabetes mellitus （T2DM）. Methods The exons 3, 4, 7, 8 of ApoJ gene were screened by polymerase chain reaction- denaturing gradient gel electrophoresis （PCR- DGGE） in 61 type 2 DM patients and 60 healthy control subjects of Chinese population. Abnormal bands were sequenced. Results The deletion/insertion pelymorphism site in exon 7 of the ApoJ gene on two subject groups had significant difference （ P 〈 0. 01 ）. Exons 3, 4, 8 in the two subject groups didn＇t have difference （ P 〉 0.05 ）. Conclusion ApeJ of exon 7 deletion/insertion pelymorphism is one of the genetic marker of 2 DM and the ApeJ polymorphism may be associated with 2 DM.
出处
《中国实用内科杂志》
CAS
CSCD
北大核心
2005年第10期897-898,共2页
Chinese Journal of Practical Internal Medicine
基金
贵州省科委科研基金项目资助计(19963036)
