摘要
目的:研究自体静脉移植后早期生长反应基因1(Egr-1)表达的动态变化,探讨其在内膜增生(IH)中的作用。方法:W istar大鼠80只,建立自体静脉移植模型。应用原位杂交和RT-PCR方法检测Egr-1 mRNA的表达,应用W estern蛋白印迹和免疫组化方法检测Egr-1蛋白表达情况,同时进行组织形态学研究。结果:自体静脉移植后,Egr-1mRNA和Egr-1蛋白的表达具有双相变化。移植1 h,Egr-1 mRNA阳性率为(35±7)%,6 h和24 h时下降,7 d时重新升高,4周达高峰(45±6)%。移植早期2 h即有Egr-1蛋白的表达,阳性率为(30±5)%,并持续至6h,24 h^3 d表达下降,移植4周Egr-1蛋白的阳性表达率达到高峰(40±9)%。结论:Egr-1的激活及表达与自体移植静脉内膜增生关系密切,Egr-1可能成为防治移植静脉内膜增生及狭窄、闭塞的一个新的干预靶点。
Objective： To investigate the development and significance of the expression of early growth response gene-1 （ Egr-1 ） in autogenous vein graft in rats and to detect the role of Egr-1 in intimal hyperplasia （ IH ）. Methods ： Autogenous vein graft model was established in 80 Wistar rats. Egr-1 mRNA was measured by RT-PCR and in situ hybridization. Western blotting and immunohistochemical method were used to detect the expression of Egr-1 protein. Results：The expression of Egr-1 mRNA and Egr-1 protein had biphasic changes. The level of Egr-1 mRNA increased rapidly 1 hour after graft, and the positive expression rate of Egr-1 mRNA was （35±7）%. Then the level declined 6 hours and 24 hours later. The level increased again 1 week later and reached the peak 4 weeks later, and the positive expression rate of Egr-1 mRNA was （45±6）%. Immunohistocbemieal staining and Western blotting revealed that Egr-1 protein expressed at the 2nd hour in early phase and that the positive expression rate of Egr-1 protein was （ 30 ± 5 ） %. The high expression continued for 6 hours. The level of Egr-1 protein decreased 24 hours and 3 days later. The positive expression rate of Egr-1 protein reached the peak of （40 ±9）% at the 4th week. Conclusion：In autogenous vein graft , Egr-1 plays an important role in the proliferation of vascular smooth muscle cells. Egr-1 may become a new target for the prevention and therapy of intimal hyperplasia, stenosis, and emphraxis after vein graft.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第5期411-413,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30400435)
辽宁省自然科学基金资助项目(2001101035)
关键词
早期生长反应基因1
移植静脉
自体
内膜增生
early growth response gene-1
vein graft
autologous
intimal hyperplasia
