摘要
目的研究三邻甲苯基磷酸酯(TOCP)诱发母鸡迟发性神经毒性(OPIDN)发生过程中坐骨神经动作电位特性的时间效应关系及其与症状分级间的关系,寻找敏感指标,为探讨TOCP引起OPIDN的发病机制及早期诊断提供依据。方法成年罗曼母鸡,一次po750mg·kg-1TOCP,在d0,5,10,15和21测定母鸡坐骨神经动作电位。结果TOCP处理母鸡逐渐出现下肢行走不协调、站立行走困难等症状,至d15完全瘫痪。坐骨神经动作电位特性随各时间点及症状分级变化。在d5,10,15和21与d0对照组相比,坐骨神经传导速度减慢,分别降低16%(P<0.05),33%,47%和47%(P<0.01);复合动作电位(CAP)潜伏期渐延长,分别增加27%(P<0.05),39%,45%和73%(P<0.01);波幅分别减小6%(P>0.05),22%,37%和40%(P<0.01);最大刺激强度分别增加10%和10%(P>0.05),31%和34%(P<0.01);阈值强度在各时间点变化不明显;痛觉阈值分别升高30%,56%,79%和80%(P<0.01)。在OPIDN症状分级为1～2,3～4,5～6和7～8级时与0级时相比,坐骨神经传导速度分别降低16%(P<0.05),33%,43%和50%(P<0.01);潜伏期分别升高27%(P<0.05),33%,40%和73%(P<0.01);波幅分别降低6%和9%(P>0.05),36%和39%(P<0.01);最大刺激强度分别升高10%和10%(P>0.05),21%和35%(P<0.01);阈强度变化不明显。结论TOCP诱导母鸡坐骨神经动作电位特性随时间延长及症状加重进行性变化,以神经传导速度及复合动作电位潜伏期变化最早、最为敏感。
AIM To investigate the early events in the development of organophosphate induced delayed neurotoxicity（OPIDN） and its mechanism through assessing the time-dependent changes in compound action potential （CAP） parameters in hen sciatic nerve treated with tri-ortho-cresyl phosphate （TOCP） and the relationship of compound action potential parameters and clinical signs. METHODS Adult Roman hens were treated with TOCP by gavage at a single dosage of 750 mg·kg^-1 The compound action potential indexes in hen sciatic nerve were determined on d 0, 5, 10, 15 and 21 post-dosing. RFSULTS TOCP-treated hens began to appear abnormal gait on d 5 with daily progression to reach total hindlimb paralysis on d 15, no recovery was observed within 21 d. Compared to the control hens （d 0）, on d 5, 10, 15 and 21 post-dosing, conduction velocity of sciatic nerve （NCV） decreased by 16% （P〈0.05）, 33%, 47% and 47% （P〈0.01）, respectively; latency of CAP were prolonged 27% （P〈0.05）, 39 %, 45 % and 73 % （P〈0.01）, respectively; amplitude （peak to peak） decreased 6% （P〉0.05）, 22%, 37% mad 40% （P〈0.01）, respectively; maximal intensity increased 10%, 10% （P〉0.05）, 31% and 34% （P〈0.01）, respectively; threshold intensity had no significant difference; pain threshold increased 30%, 56%, 79% and 80% （P〈0.01）, respectively. Compared to the control hens （clinical sign score 0）, for the groups with clinical sign scores of 1-2, 3 -4, 5-6, 7-8, NCV decreased by 16% （P〈0.05）, 33%, 43 % and 50% （P〈0.01）, respectively; latency of CAP was prolonged 27 % （P〈0.05）, 33 %, 40% and 73% （P〈0.01）, respectively; amplitude （peak to peak） decreased 6%, 9 % （P〉0.05）, 36% and 39% （P〈0.01）, respectively; maximal intensity increased 10%, 10% （P 〉 0.05）, 21% and 35 % （P〈0.01）, respectively; threshold intensity had no significant difference. CONCLUSION These results suggest that the changes in compound action potential characteristics of sciatic nerve in TOCP-treated hens be dependent on post-dosing time and progress with the clinical signs of OPIDN. NCV and latency of CAP are earlier indexes and more sensitive than others, and may be helpful to detect OPIDN early in the course of the disorder.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2005年第5期387-392,共6页
Chinese Journal of Pharmacology and Toxicology
基金
国家科技部973重大基础研究资助项目(2002CB512907)~~
关键词
三邻甲苯基磷酸酯
复合动作电位
坐骨神经
有机磷化合物
毒性
tri-ortho-cresyl phosphate
compound action potention
sciatic nerve
organophosphorus compounds
toxicity
