摘要
采用体外培养的人皮肤成纤维细胞为模型,以噻唑蓝(Thiazolylblue,MTT)染色和乳酸脱氢酶(Lactatedehydrogenase,LDH)活性为指标,探讨蒙山绿茶和老鹰茶水提取物的防长波紫外线的损伤和修复作用。结果发现,绿茶和老鹰茶水提取液对长波紫外线(UltravioletA,UVA)损伤有较强的防护作用,且呈浓度效应;在高浓度(5mg/mL)时,两种茶提取液防护作用无显著性差异,而在低浓度(2.5mg/mL和1.25mg/mL)时,同浓度的绿茶提取液的防护作用明显低于老鹰茶;绿茶、老鹰茶水提取液都对UVA损伤有修复作用,且皆呈浓度效应,两种提取液之间无显著性差异。它们对UVA辐射的这种防护和修复作用可能与茶多酚的抗氧化作用有关。
A group of cultured normal human skin-derived fibroblasts was used as the cell model to investigate protective and repair effects of aqueous extracts of Mengshan green tea and Hawk tea against 320-400nm UV-ray irradiation, with the methods of MTT colorimetry and LDH release. It was found that the aqueous extracts had strong protective effect on fibroblasts against the UV-rays with dose dependence. There were no significant differences between the two kinds of tea aqueous extracts in a higher concentration of 5mg/mL, whereas at lower concentrations of 2.5 and 1.25mg/mL the, green tea aqueous extract was less effective than the hawk-tea aqueous extract in protecting fibroblasts from the UV-ray damage. Meanwhile, it was discovered that the green tea and hawk-tea aqueous extract could repair damages induced by the UV irradiation with dose dependence. But there were no statistically significant differences between the two kinds of aqueous extract. The effects may be related to antioxidant effect of tea polyphenol.
出处
《辐射研究与辐射工艺学报》
EI
CAS
CSCD
北大核心
2005年第5期278-281,共4页
Journal of Radiation Research and Radiation Processing
基金
中国工程物理研究院军民两用基金(20040103)资助
关键词
绿茶
老鹰茶
长波紫外线
成纤维细胞
损伤修复
Green tea
Hawk-tea
Ultraviolet radiation A
Fibroblast
Damage and repair
