摘要
目的:观察罗格列酮对2型糖尿病大鼠心肌细胞病理改变的作用。方法:实验于2003-01/2003-10在河北医科大学第二医院动物室,河北医科大学电镜室、组胚教研室完成。选择健康8周龄SD雌性大鼠40只。随机选用10只大鼠为对照组:普通饲料喂养。12周时每日给予同实验组等体积的高压消毒水灌胃。给予其余30只大鼠高脂高糖饲料喂养。6周时,腹腔注射小剂量链脲佐菌素(30mg/kg,溶于0.1mol/L枸橼酸缓冲液中,pH4.4)。8周时,24只空腹血糖≥7.8mmol/L(造模成功)。12周时将24只大鼠随机分为糖尿病组和罗格列酮组各12只。罗格列酮组:每日给予罗格列酮1.42mg/(kg·d)灌胃治疗。糖尿病组:每日给予同罗格列酮组等体积的高压消毒水灌胃。24周麻醉大鼠,取出心脏,然后取部分左心室组织置于中性甲醛固定,脱水,石蜡包埋,常规切片,进行苏木精-伊红染色,光镜下观察心肌细胞病理变化,另取部分心室组织透射电镜下观察心肌细胞超微结构变化。结果:①因造模失败脱失6只,最终进入结果分析34只。②光镜可见对照组大鼠心肌细胞排列整齐,致密,结构清晰,细胞核呈卵圆形。糖尿病组大鼠心肌细胞排列紊乱,扭曲,间质有免疫细胞聚集。罗格列酮组心肌细胞排列趋于整齐,结构接近正常。③电镜可见对照组大鼠心肌细胞排列整齐,密集,细胞连接清晰。细胞核呈卵圆形,胞浆内可见大量的结构正常的线粒体和糖原。糖尿病组大鼠肌纤维排列紊乱、稀疏,线粒体肿胀,数量减少,糖原减少。罗格列酮组心肌细胞的结构基本接近正常。结论:罗格列酮可以减轻糖尿病大鼠心肌细胞的病理改变,延缓糖尿病心肌病变的发展。
AIM： To observe the effects of rosiglitazone on pathologic changes of myocardial cells in type 2 diabetic rats, METHODS： The experiment was conducted in Experimental Animal Center of the Second Hospital, Department of Electromicroscope, Department of Histology and Embryology, Hebei Medical University, from January to October 2003. A total of 40 healthy 8-week-old SD female rats were selected, Ten rats were randomly selected as control group： fed with common feed. At the 12^th week they were treated with gastric perfusion with high-pressure antiseptic solution of the equal dose of volume to the experiment group every day, Other 30 rats were given with high fat and high sugar feed, At the 6^th week, the rats were treated with streptozotocin （30 mg/kg, dissolving in the buffer of 0.1 mol/L citric acid, pH4A） through intraperitoneal injection. At the 8^th week, the fasting blood sugar ≥7.8 mmol/L （successful building models） in 24 rats. At 12^th week, 24 rats were assigned randomly into diabetes group and rosiglitazone group with 12 rats in each group. Rosiglitazone group： The rats were offered 1.42 mg/kg per day rosiglitazone with gastric perfusion every day. Diabetes group： They were provided with gastric perfusion of equal dose of high-pressure antiseptic solution like that of the rosiglitazone group every day. At 24^th week, the rats were conducted anesthesia, and the hearts were separated. Then part of the left ventricle tissue were separated and put in neutrality formaldehyde, desiccation, embedding with paraffin wax, filleting routinely, to perform the hematoxylin-eosin staining. The pathologic changes of myocardial cells were observed under the light microscope. Parts of the ventricle tissue were gained and the uhrastructure changes of myocardial ceils were observed under transmission light microscope. RESULTS： ① Six rats dropped because of the failure of building model, and 34 rats were involved in the result analysis finally. ② From the light microscope, it was showed that compact cardiac myocyte lined up in order with clear structure, and the egg-shaped nucleus in rats of the control group. The cardiac myocyte lined up disorder, distortion, and there were immunologic cells aggregation in interstitial in rats of the diabetes group. The cardiac＇myocyte was near to well arranged and the structure was close to normality in the rats of the rosiglitazone group. ③ From the electromicroscope, it was showed that in rats of the control group, the myocardial cells lined up in order, concentrated, clear cell junction. The nucleus showed oosphere-like round. There were a large number of normal structure mitochondrion and glycogen in the cell plasm. In rats of the diabetes group, the muscle fiber lined up in disorder, rarefaction, swelling of mitochondrion, and the quantity and glycogen decreased. The structure of myocardial cells was near to normality in rats of the rosiglitazone group. CONCLUSION： The rosiglitazone can relieve the pathologic changes of myocardial cells in diabetic rats, and postpone the development of the diabetic myocardial lesion.
出处
《中国临床康复》
CSCD
北大核心
2005年第31期88-89,共2页
Chinese Journal of Clinical Rehabilitation
