摘要
目的:建立莪术油葡萄糖注射液中莪术醇和吉马酮的含量测定方法。方法:高效液相色谱法。大连依利特HypersilODS2色谱柱(250mm×4.6mm,5μm),乙腈水梯度洗脱:乙腈:0～3min,45;3～30min,45→65;30～38min,65;38～45min,65→90;45～55min,90→100;55～65min,100。流速:1.0mL·min-1,柱温:25°C,检测波长:214nm。结果:莪术醇在0.26μg～6.5μg范围内线性良好(r=0.9999),吉马酮在0.1125μg～2.8125μg范围内线性良好(r=1.0000),两者的加样回收率分别为102.1、98.8(n=5)。结论:反相HPLC法能同时测定莪术油葡萄糖注射液中莪术醇和吉马酮的含量,方法简便,结果准确,可用于其质量控制。
AIM： To establish a HPLC method for determination of curcumol and germacrone in Zedoary Turmeric Oil and Glucose Injections. METHODS： RP-HPLC was applied for quantitative analysis of curcumol and germacrone. Hypersil ODS2 column （250 mm × 4.6 mm, 5 μm） and gradient elution was used. Mobile phase A was acetonitrile and mobile phase B was water. Phase A time-percentage composition was as follows：0-3 min, 45% ;3-30 min,45%→55% ;30-38 min, 65%; 38-45 min, 65%→90% ;45-55 min,90%→100% ;55-65 min, 100%. The flow rate was at 1.0 mL· min^-1. Diode array detector was set at 214 nm and column temperature was at 25 ℃. RESULTS： The calibration curves of curcumol and germacrone were linear in the range of 0.2645.5 μg （ r = 0.999 9 ） and 0.112 5-2.812 5 μg （ r = 1 ）, The average recoveries of them were 102.1% and 98.8 % （ n = 5 ）, respectively. CONCLUSION： Curcumol and germacrone can be determined by the method, and this will improve the quality control of Zedoary Turmeric Oil and Glucose Injections.
出处
《中成药》
CAS
CSCD
北大核心
2005年第10期1141-1143,共3页
Chinese Traditional Patent Medicine
关键词
HPLC
莪术油葡萄糖注射液
莪术醇
吉马酮
HPLC
Zedoary Turmeric Oil and Glucose Injections
curcumol
germacrone
