摘要
目的探讨肝素酶(heparinase)和碱性成纤维细胞生长因子(bFGF)在大肠癌组织中表达的临床意义及大肠转移和预后的关系。方法采用免疫组织化学、原位杂交方法,检测115例大肠癌石蜡切片和45例正常大肠黏膜中肝素酶和bFGF的表达情况。采用χ2检验、t检验、生存曲线和Cox比例风险回归等方法分析肝素酶和bFGF分别表达及共表达的意义。结果免疫组织化学染色证实肝素酶(91/115)和bFGF(89/115)主要表达在肿瘤细胞细胞质和(或)细胞膜中,在正常大肠黏膜上皮则呈阴性表达。肝素酶在大肠癌肿瘤细胞中表达明显增高(P=0.0001),统计结果显示肝素酶和bFGF的表达具有明显的一致性(P=0.0001),两者单独表达和共表达均与大肠癌的分期、血管浸润、淋巴结转移、微血管密度和预后有关。其中,两者共表达时与分期和微血管密度的相关性更显著;另外,bFGF还与大肠癌的分化程度有关。多因素分析结果显示大肠癌的分化程度、血管浸润、淋巴结转移和bFGF表达可以作为判断大肠癌预后的危险因素,但肝素酶不是影响预后的独立因素。结论肝素酶和bFGF均与大肠癌的转移、血管生成和预后密切相关。
Objective To assess protein and mRNA expression levels of heparinase and basic fibroblast growth factor（bFGF） genes in human cancer and their roles in tumor invasion,metastasis and prognosis. Methods A total of 115 paraffin-embedded and 45 tissue specimens of normal tissue were studied by immunohistochemisty and in situ hybridization to evaluate the protein and mRNA expression status of heparinase and bFGF genes,the data were analyzed by SPSS statistical software. Results Both human heparinase and bFGF were highly expressed in cancer cell,in contrast to none or a low expression in normal tissue.Expression of heparinase also showed a significantly higher level than it did in the normal tissue（P=0.041 ）.Immunohistochemistry showed that heparinase expression was both cytoplasmic and membranous.The agreement between heparinase and bFGF was significant.Either uniexpression or coexpression of the heparinase and bFGF was always related to colorectal cancer＇s staging,vascular infiltration,lymphatic metastasis,microvascular density （MVD） ,and the prognosis.Coexpression of the two protein demonstrated an even higher correlation with the tumor staging and MVD.In addition expression of bFGF correlated with tumor cell differentiation. Data of a multivariate analysis indicated that tumor cell differentiation,vascular invasion,lymphatic metastasis and expression of bFGF were identified as significant prgonstic parameters. Conclusion Both heparinase and bFGF may play important roles in tumor angiogenesis,metastasis and prognosis of colorectal cancer.
出处
《中国药物与临床》
CAS
2005年第10期742-746,共5页
Chinese Remedies & Clinics
基金
山西省自然科学基金资助项目(2004-42)
太原市科学技术基金资助项目(2004-72)
