摘要
目的检测刺鼠信号蛋白对自体移植皮片中酪氨酸酶的活性影响,进一步认识自体移植皮片过度色素沉着的原因。方法建立过度色素沉着的自体移植皮片的动物模型;利用免疫组织化学方法分别检测刺鼠信号蛋白作用前后自体移植皮片中酪氨酸酶的表达,并与对照治疗组及正常皮肤相比较。结果酪氨酸酶的表达定位于表皮基底部黑色素细胞的胞浆,在大部分组织中呈阳性表达,经刺鼠信号蛋白作用后酪氨酸酶在自体移植皮片中的表达明显减少,与在其他各对照组中的表达差异有极显著意义(P<0.01)。结论刺鼠信号蛋白在自体移植皮片中能竞争性拮抗α-MSH的黑色素合成,使皮片着色能力降低,从而证明皮片移植后表皮细胞中α-MSH的表达上调是皮片呈过度色素沉着的重要原因。
Objective To observe the influence of tyrosinase activity in skin autografts by agouti signal protein, and elucidate the cause of hyperpigmention in skin autografts. Methods To establish the animal model of hyperpigmented skin autografts. Immunohistochemical technique was utilized to detect the expression and distribution of tyrosinase protein in skin autografts on animal model before and after ASIP trearnent and normal skin respectively, Statistics was used to analyze the data. Results The expression of tyrosinase was situated in kytoplasm of epidermal melanocyte. The expression of tyrosinase in skin autografts after ASIP treatment was downward regulated, as opposed to that of control group. Conclusion The results indicate that ASIP can antagonize the melanogenic effect of α- MSH, with a reduction of pigmentation in skin autografts. The results further elucidate that overexpression of α- MSH in epidermal cell after skin graft is a significant cause of hyperpigmentation in skin autografts.
基金
国家自然科学基金资助项目(30371473)
