摘要
采用磷酸苯二钠法在520nm波长测定了未成熟、早熟和正常成熟中华绒螯蟹肝胰腺与性腺中的酸性磷酸酶(ACP)和碱性磷酸酶(ALP)。结果表明:(1)各组蟹肝胰腺ACP和ALP活性都显著高于性腺;(2)早熟蟹肝胰腺中ACP活性都显著高于未成熟蟹而低于正常成熟蟹,在各发育阶段没有性别差异,但是,早熟雌蟹和正常成熟雌蟹卵巢中ACP活性都较早熟雄蟹和正常雄蟹精巢低,早熟与正常成熟个体没有差异;(3)早熟雌蟹肝胰腺ALP活性显著高于未成熟和正常成熟雌蟹,而与三组雄蟹处于同一水平,但是,早熟和正常成熟雌雄蟹的性腺中ALP活性没有显著差异。由此得出结论:(1)性腺发育所需营养物质的动员或合成主要与肝胰腺ACP有关;(2)性早熟的发生与肝胰腺中ACP和ALP活性的显著升高有关。
The activities of ACP and ALP in hepatopancreas and gonads of immature, precocious and normally mature Eriocheir sinensis were assayed at 520nm wave length. Firstly, the activities of ACP and ALP in hepatopancreas were significantly higher than those in gonads. Secondly, the activities of ACP in hepatopancreas of precocious crabs were significantly higher than those of immature crabs, but lower than those of normally mature crabs, and no sexual difference existed. However, the activities of ACP in ovaries of precocious and normally mature female crabs were correspondingly lower than those in testes of precocious and normally mature male crabs. There was no difference between precocious and normally mature crabs. Thirdly, the activities of ALP in hepatopancreas of precocious female crabs were significantly higher than those of immature and normally mature female crabs, but at the same level as three groups of male crabs. However, there was no significantly difference between the activities of ALP in gonads of precocious and normally mature crabs. Therefore, the following could be concluded： （1）mobilization or synthesis of those nutrients required by gonads was related to ACP in hepatopancreas; （2）onset of precocity was related to significant elevation of activities of ACP and ALP.
出处
《水产学报》
CAS
CSCD
北大核心
2005年第5期630-634,共5页
Journal of Fisheries of China
基金
上海市教委重点学科资助项目"中华绒螯蟹性早熟机理的研究"(B991602)
上海市重点学科建设项目资助(Y1101)
关键词
中华绒螯蟹
肝胰腺
性腺
性早熟蟹
磷酸酶
Eriocheir sinensis
hepatopancreas
gonad
precocious crab
phosphatase
