摘要
目的:探讨夜来香根茎提取物对小鼠的镇痛作用及其机制,为临床(骨科)疼痛治疗寻找新药。方法:实验于2005-03/04在赣南医学院科研中心实验室完成。①小鼠(n=40),随机分为4组,每组10只,分别腹腔注射0.02mL/g生理盐水,夜来香根茎提取物0.1mg/g,0.2mg/g,0.1mg/g氨基比林,15min后腹腔注射6g/L冰醋酸0.01mL/g,观察记录给药后15min内小鼠扭体次数。②雌性小鼠(n=40),随机分为4组,每组10只。分别腹腔注射0.02mL/g生理盐水,0.1,0.2mg/g夜来香根茎提取物,0.01mg/g吗啡,用热板法测定小鼠15,30,60min痛觉反应。③雌性小鼠(n=30),随机分为3组,每组10只,分别腹腔注射0.02mL/g生理盐水,0.004mg/g纳洛酮+0.01mg/g吗啡,0.004mg/g纳洛酮+0.1mg/g夜来香根茎提取物,用热板法测定小鼠15,30,60min痛觉反应。④小鼠(n=40),随机分为4组,每组10只,分别给予夜来香根茎提取物0.1mg/g,0.2mg/g及1g/L的吗啡和生理盐水于20,35,50,70min重复电刺激,用电刺激法测定痛觉反应。结果:各实验组小鼠无脱失情况。在热板法致痛作用实验中如有小鼠跳出给予排除。①小鼠扭体反应次数:0.1mg/g,0.2mg/g夜来香根茎提取物及0.1mg/g氨基比林对醋酸诱发小鼠扭体反应有非常显著的镇痛作用,给药后扭体反应次数均少于生理盐水组(20.2±10.8,14.5±7.6,7.6±4.5,50.6±15.5,P<0.01),且夜来香根茎提取物的镇痛效果呈剂量依赖性。②热板法致小鼠痛觉反应的时间:0.1mg/g,0.2mg/g夜来香根茎提取物对热板致痛有显著的镇痛作用,给药后15,30,60min痛觉反应的时间均长于生理盐水组[(22.1±6.2),(24.6±8.8),(22.9±8.6)s;(26.2±8.0),(31.1±10.3),(29.4±8.7)s;(12.1±3.1),(11.9±2.8),(12.8±3.0)s,P<0.05～0.01],且呈剂量依赖性。纳洛酮0.004mg/g+夜来香根茎提取物0.1mg/g组给药后各时间点痛觉反应的时间均长于生理盐水组[(28.3±6.9),(22.4±5.8),(20.1±5.5)s;(12.5±3.1),(12.8±2.8),(12.1±3.0)s,P<0.05～0.01]。纳洛酮0.004mg/g+吗啡0.01mg/g组给药后各时间点痛觉反应的时间[(13.1±3.3),(12.9±3.1),(13.2±2.8)s]与生理盐水组相比接近。③电刺激法致小鼠镇痛率:0.1mg/g,0.2mg/g夜来香根茎提取物及吗啡组给药后20,35,50,70min镇痛率均高于生理盐水对照组[(45±5)%,(40±10)%,(35±5)%,(20±5)%;(85±15)%,(80±5)%,(60±10)%,(30±5)%;(90±10)%,(85±10)%,(75±15)%,(45±15)%;0,P<0.01]。结论:夜来香根茎提取物具有明显的镇痛作用,其镇痛强度与氨基比林相当,且呈剂量依赖性。阿片受体拮抗剂纳洛酮可拮抗吗啡的镇痛作用,但阿片受体拮抗剂纳洛酮不能拮抗夜来香根茎提取物对热板致痛法小鼠的镇痛作用,表明夜来香根茎提取物的镇痛作用机制并非激动阿片受体而镇痛的。
AIM： To study the analgesic effect of Cestrum noctumum L. （CNL） extract in mice and its mechanism, and look for new drug for clinical （department of orthopaedics） ache treatment. METHODS： The experiment was conducted at the central laboratory of science research, Gannan Medical College from March to April 2005. ① Mice （n=40） were divided randomly into 4 groups with 10 in each group, and were treated with 0.02 mL/g saline, 0.1 mg/g,0.2 mg/g CNL extract and 0.1 mg/g aminopyrazolin through intraperitoneal injection, respectively. After 15 minutes, the mice were treated with intraperitoneal injection with 0.01 mL/g glacial acetic acid 6 g/L. The number of times of twisting body in mice were observed and recorded within 15 minutes.② Female mice （n=40） were assigned randomly into 4 groups with 10 mice in each group, and were treated with 0.02 mL/g saline, 0.1,0.2 mg/g CNL extract and 0.01 mg/g morphina through intraperitoneal injection, respectively. The reaction of pain perception was detected with hot-plate method at 15, 30 and 60 minutes. ③ Female mice （n=30） were divided randomly into 3 groups with 10 in each group, and were treated with 0.02 mL/g saline, 0.004 mg/g naloxone ＋ 0.01 mg/g morphina）, 0.004 mg/g maloxone ＋ 0.1mg/g CNL extract through intraperitoneal injection, respectively. The reaction of pain perception was detected with hot-plate method at 15, 30 and 60 minutes. ④ Mice （n=40） were assigned randomly into 4 groups with 10 mice in each group, and were treated with 0.1 mg/g,0.2 mg/g CNL extract, 1 g/L morphina and saline, respectively, and at the 20,35,50 and 70 minutes to repeat the electric stimulation. The reaction of pain perception was tested with electric stimulus method. RESULTS： There was no drop in every experimental group. The mice that had dapped out during the experiment with hot-plate method were excluded. ① The number of times of twisting body reaction in mice： The analgesic effect on the twisting body due to acetic acid was very significant with 0.1 mg/g,0.2 mg/g CNL extract and 0.1 mg/g aminopyrazolin. The number of times of twisting body after adiministration were all less than that in the saline group （20.2±10.8,14.5±7.6, 7.6±4.5, 50.6±15.5, P〈0.01 ）, and the analgesic effect of CNL extract showed dose-dependent effects. ② Time of pain perception reaction due to hot-plate method in mice： The 0.1 mg/g,0.2 mg/g CNL extract had significant analgesic effect on pain induced by hot-plate. After administration, the time of pain per- ception reaction at 15,30,60 minutes was longer than that in the saline group [（22.1±6.2）, （24.6±8.8）, （22.9±8.6）s; （26.2±8.0）, （31.1±10.3）, （29.4±8.7） s; （12.1±3.1）, （11.9±2.8）, （12.8±3.0）s, P〈 0.05-0.01] and showed dose-dependent effects. The reactive time of pain perception in every time point in the 0.004 mg/g naloxone ＋ 0.10 mg/g CNL extract group after administration was longer than that in the saline group [（28.3±6.9）, （22.4±5.8）, （20.1±5.5） s; （12.5±3.1）,（12.8±2.8）, （12.1±3.0） s, P 〈 0.05-0.01]. The reactive time of pain perception in every time point in the 0.004 mg/g naloxone ＋ 0.01 mg/g morphina group after administration [（13.1±3.3）, （12.9±3.1）, （13.2±2.8） s] was near to that in the saline group. ③ Analgesia rate in mice with electric stimulation： The analgesia rate was higher in the 0.1mg/g,0.2mg/g CNL extract and at 20,35,50,70 minutes after administration in .the morphina group than that in the saline control group [（45±5） %, （40±10） %, （35±5） %, （20±5） %; （85±15） %, （80±5） %, （60±10） %, （30±5） %; （90±10） %, （85±10） %, （75±15） %, （45±15） %; 0, P〈 0.01]. CONCLUSION： The CNL extract has obvious analgesic effect and its analgesic intensity is similar to that of aminopyrazolin, and showed dosedependent effects. The apiate receptor antagonist, naloxone can antagonize the analgesic effect of morphine, but cannot antagonize the analgesic effect of CNL extract on pain due to hot plate. It expresses that the analgesic effect of CNL extract does not ease pain by agitating the opiate receptor.
出处
《中国临床康复》
CSCD
北大核心
2005年第37期35-37,共3页
Chinese Journal of Clinical Rehabilitation
