摘要
目的:探讨半胱氨酸天冬氨酸蛋白酶3的分子生物学研究进展及它在中枢神经系统疾病中的作用。资料来源:应用计算机检索Medline数据库1995-01/2005-02相关文章,检索词为“caspase-3”,“apoptosis”,“centernervesystem”,“emergen-cydiseases”分别组合进行检索,限定文章语言种类为English。资料选择:对资料进行初审,选取与半胱氨酸天冬氨酸蛋白酶3有关的分子生物学方面的文章和半胱氨酸天冬氨酸蛋白酶3与中枢神经系统疾病有关的随机对照实验。资料提炼:共收集到156篇关于半胱氨酸天冬氨酸蛋白酶3与中枢神经系统有直接关系的文章,其中有25篇符合标准,排除131篇。排除的131篇系重复或者与本文关系不大。资料综合:对检索到的文章的相关信息综合加以概括综述,总结出在急性脑损伤、急性脊髓损伤、脑缺血、脑出血后存在半胱氨酸天冬氨酸蛋白酶3活性增高,应用广谱半胱氨酸天冬氨酸蛋白酶抑制剂Z-VAD-fmk可显著降低半胱氨酸天冬氨酸蛋白酶3活性。结论:半胱氨酸天冬氨酸蛋白酶3是凋亡过程中最重要的蛋白酶,是多种凋亡途径的共同下游效应部分,是细胞凋亡蛋白酶级联反应的必经之路。半胱氨酸天冬氨酸蛋白酶3激活后可裂解细胞内固有的和保护性酶类,进而形成细胞凋亡的形态学及生物化学特征性改变。DNA片段化是急性脑损伤早期神经细胞凋亡的重要生化特征,DNA片段化的形成是必需的。
OBJECTIVE： To probe into the progression of biochemical study on caspase-3 and its functions in disorders of center nerve system, DATA SOURCES：The relevant papers were looked up on Medline database from January 1995 to February 2005 with the retrieved words as “caspase-3”, “apoptosis”, “center nerve system” and “emergency diseases” in combination, limited in English version. STUDY SELECTION： By initial checking, the papers on caspase-3 in molecular biology and randomized control experiments on caspase-3 and the disorders in center nerve system were collected. DATA EXTRACTION： Totally 156 papers were collected directly related to caspase-3 and center nerve system, of which, 25 papers were in conformity with the criteria and 131 papers were exuded because of repeated contents and indirect relation with this paper. DATA SYNTHESIS： To synthesize the relevant information from the retrieved papers and sum up that the activity of caspase-3 is increased after acute cerebral injury, acute spinal injury, cerebral ischemia and cerebral hemorrhage and broadspectrum caspase-3 inhibitor, Z-VAD-fmk can reduce significantly the activity of caspase-3. CONCLUSION： Caspase-3 is the most important protease in cell apoptosis, it is the component in downstream effect of coordination of various apoptotic approaches and is the only way in protease cascade of cell apoptosis. The activated caspase-3 can split the essential and protective enzymes in cell and result in the alternations of apoptosis in morphology and biochemistry. DNA segmentation is the important biochemical property of nerve cell apoptosis at early stage in acute cerebral injury and the formation of it is necessary.
出处
《中国临床康复》
CSCD
北大核心
2005年第37期190-192,共3页
Chinese Journal of Clinical Rehabilitation
