摘要
目的:建立加替沙星的反相高效液相色谱分析方法,并对其肝损害模型大鼠体内过程进行分析研究。方法:健康和肝损害造模大鼠口服20m g/kg加替沙星后5m in、15m in和4h测定各组织药物浓度,大鼠口服加替沙星后收集尿液、胆汁与粪便,测定累积、排泄率。结果:健康和肝损害造模大鼠口服20m g/kg加替沙星后5m in、15m in和4h快速分布在各组织中,其中肝、肾、小肠、胃分布最多,大脑未测到药物。大鼠口服20m g/kg加替沙星后48h尿液、胆汁与粪便药物累积及排泄率分别为(66.2±8.8)%与(71.2±13.6)%、(8.05±3.08)%与(1.62±0.67)%、(3.63±1.65)%与(3.92±1.87)%。结论:加替沙星在肝损害模型大鼠组织分布和尿液、粪便的排泄未受到影响,胆汁排泄存在统计学差异,通过了解加替沙星体内过程,可为临床应用提供参考。
Objective： To establish HPLC method for determination of gatifloxacin, analyzing in vivo course of rats. Methods：After a single dose of 20mg/kg gatifloxacin to normal and liver damaged rats, tissues of drug concentrations at 5 min,15 rain and 4 h were determined. The urinary, bile and dejection accumulative excretions were determined after a dose of 20mg/kg gatifloxacin to normal and liver damaged rats. Results ：After a single dose of 20mg/kg gatifloxacin to normal and liver damaged rats, tissues of drug concentrations at 5 min, 15 min and 4 h was rapidly and extensively distributed to all tissues, especially in liver, intestines, stomach and kidney. Gatifloxacin was not determined in brains. The urinary, bile and dejection accumulative excretions in rats were （66.25±8.8）% and （71.25±13.6 % ）, （8. 055±3.08）% and （1.62 5±0. 67）%, （3.63 ± 1.65）% and （3.92 5±1.87）%. Conclusion ：Gatifloxacin is influenced in tissues distribution and urinary and dejection to liver damaged rats. The bile excretion of gatifloxacin shows no significant difference. Understanding in vivo course of gatifloxacin will offer the reference for the clinical application.
出处
《华夏医学》
CAS
2005年第6期896-899,共4页
Acta Medicinae Sinica
基金
广西自然科学基金资助(编号:0249016)
关键词
加替沙星
体内过程
组织分布
肝损害
gatifloxacin
in vivo course
tissues distribution
liver damaged
