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人表皮角朊细胞无血清培养的实验研究 被引量:2

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摘要 采用IMDM培养基配以10%无血清培养基血清替代物(牛血清白蛋白、人转铁蛋白、胰岛素、胆固醇、过氧化氢酶),添加表皮细胞生长因子、霍乱毒素、氢化可的松、乙醇胺组成无血清培养体系进行人表皮角朊细胞培养。结果表明:表皮角朊细胞贴壁和生长良好。细胞倍增时间4.46d,增殖活性第5~10d为最高,培养第5d增殖活性与有血清培养体系比较无明显差异,提示该无血清培养体系适合于人表皮角朊细胞体外培养。
出处 《江西医药》 CAS 1996年第2期69-71,共3页 Jiangxi Medical Journal
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