摘要
利用N87-1(耐旱)×9526(敏感)的F2分离群体,构建了包含103个SSR标记的连锁图谱;在干旱胁迫与正常灌溉两种环境下调查了183个F2:3家系的抽雄期、吐丝期及抽雄-吐丝间隔等3个开花期性状;然后选用复合区间法对控制这些性状的QTL进行了鉴定。结果表明:抽雄期在胁迫环境下检测到1个QTL位于第7连锁群上,单个QTL可解释表型变异的14.89%;在正常灌溉下检测到4个QTL分别位于第1、4、7和第9连锁群上,累计可解释表型变异的44.3%。吐丝期在胁迫环境下鉴定出1个QTL,位于第7连锁群上,占表型方差的15.80%,与胁迫环境下的抽雄期QTL的位置、加性效应方向和基因作用方式一致;在正常环境下检测到2个QTL,分别位于第4和7连锁群上,累计可解释表型变异的20.56%。ASI在胁迫环境下检测到1个QTL位于第9连锁群上,单个QTL可解释表型变异的8.88%;在正常环境下检测到了2个QTL,均位于第6连锁群上,累计可解释表型变异的17.2%。经比较分析,第7连锁群上所与标记umc1015连锁的QTL具有重要的标记辅助选择利用潜力。所鉴定QTL的基因效应以部分显性为主。
A genetic map based on a maize（Zea mays L） F2 population consisting of 183 F2 individual from the cross 87-1 （drought tolerance） × 9526（drought sensitive ）, was constructed with 103 simple sequence repeat（SSR） markers. The flowering parameter including male flowering （MF）, female flowering（FMF） and anthesis-silking i nterval（ASI） were evaluated under drought stressed （DS） and nonstress（CK） environment. Quantitative trait location （QTLs） of these traits were identified by composite interval mapping.The results showed that One QTL involved in the expression of MF was identified on chromosome 7 under DS, accounting for 14.89 % of the phenotypic ;four QTLs of MF were detected on chromosome 1, 4 and 7 respectively under CK, together accounting for 44.3 % of the phenotypic variance; For FMF, one QTL was identified on chromosome 7 under DS,which was similar to MF（DS） QTL with chromosome position and direction of additive genetic effect ; 2 QTLs were detected on chromosome 4 and 7 respectively under CK and could explain 20.56 % of the phenotypic variance;. Two QTLs controlling ASI were both detected on chromosome 6 under CK, accounting for 17.2 % of phenotypic variance totally;One QTL was identified on chromosome 9 and could explain 8.88 % of phenotypic variance. QTL locating on chromosome 7 and linkaged with marker umc1015 had great potentinal value for molecular assistant selection. Most of these QTLs＇ gene effect were partial dominant.
出处
《西南农业学报》
CSCD
2005年第5期593-597,共5页
Southwest China Journal of Agricultural Sciences
基金
四川省科技攻关项目(04NG002-006-2)
亚洲玉米生物技术网项目资助
关键词
玉米
QTL
开花期
干旱胁迫
maize（ Zea mays L. ）
drought tolerance
QTL
SSR
