摘要
为了解南京地区戊型肝炎病毒(Hepatitis E virus,HEV)基因型的分布以及变异状况,本研究采用逆转录套式聚合酶反应(RT-nPCR)的方法检测南京地区40份急性散发性戊型肝炎患者的粪便标本,对PCR产物进行测序,利用生物信息学软件比较核苷酸的同源性、遗传距离,进行基因分型和变异分析。40份粪便标本中检测到14份阳性HEV、RNA,检出率为35%,基因分型均为HEV IV型,且分属2个不同的亚型;利用生物信息学软件对国内I型和IV型毒株加以比较,发现HEV IV型毒株比I型变异程度高,不同年份的HEV IV型毒株变异更大,有新的亚型出现,且变异有随时间推移而增大的趋势。
The objective of this study was to identify and analyze genotype distribution and genetic variation of Hepatitis E virus （HEV） isolated in Nanjing, China. RT-nPCR based on universal primers for multiple HEV genotypes was used to detect HEV RNA in 40 samples collected from patients with acute sporadic hepatitis E. PCR products were sequenced and analyzed with the LASERGENE and PHYLIP softwares. Fourteen out of the 40 samples were positive for RT-nPCR. The positive rate was 35%. All of the fourteen isolates werc clustered into HEV genotype Ⅳ and were separated into two different subgenotypes. The nucleotidc sequences of the HEV isolates of genotype Ⅳ presented higher variability when compared with the sequences of the Chinese isolates of HEV genotype Ⅰ. Moreover, a new subtype of HEV genotype Ⅳ was discovered in this study.
出处
《中国病毒学》
CSCD
2005年第5期468-471,共4页
Virologica Sinica
基金
国家自然科学基金(30271231
30271212)
江苏省自然科学基金(KB2002053)
江苏省社会发展科技指导性计划(BS2004506)资助项目
