摘要
采用农杆菌介导法将外源三价抗虫基因(Bt-CpTI-GNA)导入常规棉花品种中,获得转基因再生株,分子检测表明外源基因已在棉花体内表达,并遗传给后代材料。PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。其所携带的基因在转基因棉花中有分离现象,这可能是外源基因整合到受体棉株体内“基因沉默”而引起所致。
Foreign genes （Bt-CpTI-GNA） resistant to cotton insects were introduced into conventional cotton cultivars by Agrobacterium tumefaciens-mediated transformation. Regenerated plants have been obtained. The results of molecular detection indicated that the foreign genes had been transferred into cotton plants. The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance were quite regular. Some of their progenies＇ segregation coincided with Mendel＇s regularity of segregation, but some deviated. This phenomenon may be due to ＂gene silence＂.
出处
《棉花学报》
CSCD
北大核心
2005年第6期353-359,共7页
Cotton Science
基金
国家"863"项目(2004AA212102)
山西省科技攻关项目(021010)
山西省自然科学基金项目(20011091)
