摘要
利用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）从大鼠脑组织中钓得神经肽Ｙ前体ｃＤＮＡ编码区序列，将该ｃＤＮＡ定向亚克隆入哺乳动物细胞表达载体ｐＲｃ／ＣＭＶ中，构建了重组质粒ｐＲｃ／ＣＭＶ－ＮＰＹ。经双脱氧核苷酸终止法对插入片段作序列分析，证明ＮＰＹｃＤＮＡ序列的准确性及插入方向正确。
Rat neuropeptide Y precursor cDNA coding sequence was amplified by RT-PCR from rat brain tissue and then subcloned into a mammalian expression vector pRc/CMV at the Hind Ⅲ and XbaⅠsites to construct the recombinant plasmid pRc/CMV-NPY. The insert fragment was identificated by endonucleases digest and sequenced to confirm the specific NPY precursor coding region fragment.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1996年第2期69-71,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
