摘要
目的观察骨形态发生蛋白-2(BMP-2)基因修饰的组织工程骨修复节段性骨缺损效果及异种骨支架体内应用的安全性。方法①制备去抗原牛松质骨块(BCB),植入小鼠股四头肌袋内,术后行淋巴细胞转化试验和组织学观察。②在腺病毒载体介导下将BMP-2基因导入兔骨髓间质干细胞后,种植到BCB支架中,构建基因修饰的组织工程骨。于兔双侧桡骨中段造成15mm骨缺损,采用5种方法进行处理BMP-2基因转染细胞+BCB(A组);未转染细胞+重组BMP-2+BCB(B组);对照基因转染细胞+BCB(C组);未转染细胞+BCB(D组);单纯BCB(E组)。术后4、8、12周行X线、组织学和生物力学检测。结果①BCB具有较低的抗原性和良好的组织相容性;②A组术后4周诱导生成软骨组织并向编织骨转化,12周骨缺损修复,髓腔再通,新骨强度明显优于其他各组(P<0.01)。结论BMP-2基因修饰的组织工程骨是修复节段性骨缺损的好方法。
Objective： To observe the effects of BMP-2 gene modified tissue engineering bone in the treatment of segmental bone defects and the safety of xenogenetic scaffolds in vivo. Methods： （1）Antigen-free bovine cancellous bone （BCB） was prepared and implanted into the quadriceps femoris of mice. Lymphocytes proliferation assay and histological observation were done after operation. （2）The rabbit bone mesenchymal stem cells （BMSC） were transfected by BMP-2 gene which mediated by adenovirus vector （Ad-BMP-2）, then was seeded on the BCB scaffolds to create gene modified tissue engineering bone. Bone defects of 15 mm were created on the bilateral radius of rabbits and treated with five kinds of implantations：Ad-BMP-2 infected BMSC with B CB （Group A）; BMSC-BCB with recombined hBMP-2 （Group B）;Ad-Lacz infected BMSC-BCB （Group C） ;BMSC-BCB（Group D） and only BCB scaffolds （Group E）. After 4,8 ,and 12 weeks of the operations, X-ray, histological examination and biomechanics analysis were conducted. Results： ①BCB had low antigenicity and fine histocompatibility; ②In group A, cartilage formation was observed and transformed into woven bone after 4 weeks. Bone defects healed and marrow cavity opened again after 12 weeks. The new bone strength was obviousty superior to that of other groups （ P 〈 0.01 ）. Conclusion： BMP-2 gene modified tissue engineering bone is good to repair segmental bone defects.
出处
《中国骨伤》
CAS
2005年第10期601-603,共3页
China Journal of Orthopaedics and Traumatology
基金
国家自然科学基金项目(39800151)
吉林省科技厅基金项目(20010110)
关键词
骨形态发生蛋白质类
基因疗法
组织工程
骨再生
免疫学
Bone morphogenetic protein
Gene therapy
Tissue engineering
Bone regeneration
Immunology
