摘要
目的:观察具有清热、镇惊、理湿、消肿、镇痛等功效的中药拳参正丁醇提取物对大鼠心肌缺血再灌注损伤的保护作用。方法:实验于2004-09/2005-12在赣南医学院科研中心实验室完成。①取32只雄性Wistar大鼠,随机分为4组,每组8只,分别为假手术组、缺血再灌注组、拳参正丁醇提取物实验样品由沈阳药科大学植化教研室邱峰博士提供.高剂量组(120mg/kg)、拳参正丁醇提取物低剂量组(60mg/kg)。结扎冠状动脉左前降支使心肌缺血,40min后恢复血流并持续120min,复制缺血再灌注损伤模型。治疗组于结扎冠状动脉左前降支前20min经十二指肠注入拳参正丁醇提取物。缺血再灌注组及假手术组经十二指肠注入等体积生理盐水。按南京建成生物工程研究所提供试剂盒说明测定血清乳酸脱氢酶、磷酸肌酸激酶值。测定心肌组织丙二醛和超氧化物歧化酶值。用硫代巴比妥法测定丙二醛含量,用羟胺法测定超氧化物歧化酶值。②另取24只Wistar大鼠,分别为缺血再灌注组、拳参正丁醇提取物高剂量组(120mg/kg)、拳参正丁醇提取物低剂量组(60mg/kg)。同上述动物模型制作方法进行实验。以梗死区心肌湿质量占全心肌湿质量的百分比表示心肌梗死的范围。③用t检验进行组间计量资料差异比较。结果:大鼠56只均进入结果分析。①心肌组织超氧化物歧化酶含量:缺血再灌注组和拳参正丁醇提取物高低剂量组均明显低于假手术组(t=5.27～12.15,P<0.01);拳参正丁醇提取物高剂量组明显高于缺血再灌注组(t=2.30,P<0.05)。②心肌组织丙二醛含量:缺血再灌注组和拳参正丁醇提取物高低剂量组均明显高于假手术组(t=9.00～10.25,P<0.01);拳参正丁醇提取物高剂量组明显低于缺血再灌注组(t=1.97,P<0.05)。③血清乳酸脱氢酶和磷酸肌酸激酶活性:缺血再灌注组和拳参正丁醇提取物高低剂量组均明显高于假手术组(t=2.93～18.36,P<0.01),拳参正丁醇提取物高低剂量组明显低于缺血再灌注组(t=1.99～6.99,P<0.05～0.01)。④心肌梗死范围:拳参正丁醇提取物高、低剂量组大鼠均明显小于缺血再灌注组[(34.24±1.51)%,(34.93±1.88)%,(38.66±3.89)%,t=2.99,2.43,P<0.01,0.05],尤以高剂量组明显。结论:拳参正丁醇提取物通过提高心肌组织的超氧化物歧化酶和降低丙二醛,降低血清乳酸脱氢酶和磷酸肌酸激酶,清除自由基,防止脂质过氧化而产生保护心肌的作用,该作用随着拳参正丁醇提取物剂量增大而增强。
AIM： To observe the protective effect of the traditional Chinese medicine of polygonum bistora L. n-butyl alcohol extract （PBNA）, which has the effects of reducing fever, relieving convulsion, regulating dampness, subducing swelling and analgesia, on myocardial ischemia/repeffusion injury in rats. METHODS： The experiment was completed in the laboratory of scientific research center of Gannan Medical College between September 2004 and December 2005. ① Thirty-two male Wistar rats were randomly divided into 4 groups with 8 rats in each group： sham-operated group, ischemia repeffusion group, PBNA high （120 mg/kg） and low （60 mg/kg） dosage groups. Rat models of ischemia/repeffusion inju~ were duplicated by ligating left anterior descending coronary artery for 40 minutes followed by reperfusion for 120 minutes. The two PBNA groups were treated with PBNA （provided by Doctor Qiu Feng of Shenyang Pharmaceutical University） respectively by given the drugs via duodenum at 20 minutes before ischemia repeffusion, and those in the ischemia repeffusion group and shamoperated group were given saline instead. The activities of serum lactate dehydrogenase and creatine phosphokinase were detected with the kit provided by Nanjing Jiancheng Bioengineering Institute, the content of malondialdehyde and activity of superoxide dismutase in myocardial tissue were detected with thiobarbituric acid （TBA） method and hydroxylamine method respectively.② Another 24 Wistar rats were divided into ischemia reperfusion group, PBNA high （120 mg/kg） and low （60 mg/kg） dosage groups. The model establishments were the same as those mentioned above. The myocardial infarcted range was expressed with the percentage of myocardial wet mass in the infarcted area to that of the total myocardium. ③The t test was applied to compare the differences of the measurement data among the groups. RESULTS： All the 56 rats were involved in the analysis of results. ① The activity of superoxide dismutase in myocardial tissue： It was obviously lower in the ischemia reperfusion group and PBNA high and low dosage groups than in the sham-operated group （t=5.27-12.15, P 〈 0.01）, but obviously higher in the PBNA high dosage group than in the ischemia reperfusion group （t=2.30, P 〈 0.05）. ②The content of malondialdehyde in myocardial tissue： It was obviously higher in the ischemia repeffusion group and PBNA high and low dosage groups than in the sham-operated group （t=9.00-10.25, P 〈 0.01）, but obviously lower in the PBNA high dosage group than in the ischemia reperfusion group （t=1.97, P 〈 0.05）.③ The activities of serum lactate dehydrogenase and creatine phosphokinase： Those were obviously higher in the ischemia reperfusion group and PBNA high and low dosage groups than in the sham-operated group （t=2.93-18.36, P〈 0.01）, but obviously lower in the PBNA high and low dosage groups than in the ischemia reperfusion group （t=1.99-6.99, P 〈 0.05-0.01）. ④Range of myocardial infarction： It was obviously smaller in the ischemia reperfusion group and PBNA high and low dosage groups than in the sham-operated group [（34.24±1.51）%, （34.93±1.88）%, （38.66±3.89）%, t=2.99, 2.43, P 〈 0.01, 0.05], especially in the high dosage group. CONCLUSION： Polygonum bistora L. n-butyl alcohol extract has obvious protective effects on myocardium in a dosage-dependent manner by increasing the superoxide dismutase and decreasing malondialdehyde in myocardial tissue, decreasing serum lactate dehydrogenase and creatine phosphokinase, eliminating free radicals, and preventing lipid peroxidation.
出处
《中国临床康复》
CSCD
北大核心
2005年第39期118-120,共3页
Chinese Journal of Clinical Rehabilitation
基金
江西省卫生厅中医药管理局科研基金课题(2003A33)~~
