摘要
目的研究中药金银花水提物(以下简称金银花)对卵清蛋白(OVA)致敏小鼠的免疫调控作用,探索中药治疗食物过敏的可行性。方法取BALB/c小鼠40只,均分为5组,每组8只,按照肠道激发后灌服不同浓度的金银花水提物分为100 mg/100 m l(H)、50 mg/100 m l(M)、25 mg/100 m l(L)浓度组,激发后不给药组(Ch),以及正常生理盐水(NS)对照组。取空肠行HE及甲苯胺蓝染色;组织荧光法测定小肠组胺含量;ELISA法测定外周淋巴组织单个核细胞(PLNMC)培养上清液中白细胞介素4(IL-4)、γ干扰素(IFN-γ)及血清OVA特异性IgE(OVA-sIgE)水平;RT-PCR测定PLNMC中IL-12p40 mRNA表达;足垫肿胀实验检测迟发型超敏反应。结果H、M组浓度的金银花水提物可缓解过敏小鼠小肠绒毛炎症,减轻肥大细胞聚集和脱颗粒,提高固有层完整肥大细胞比率,减轻过敏小鼠肠道组胺释放,降低过敏小鼠体内IL-4、OVA-sIgE水平及IL-4/IFN-γ比值,抑制PLNMC中IL-12 mRNA表达;三种浓度金银花可缓解OVA介导的小鼠足垫迟发性超敏反应(DTH)。结论证实金银花水提物可参与OVA致敏小鼠的免疫调控,对缓解OVA介导的小鼠IgE及细胞介导的变态反应有利,用于食物过敏治疗具有潜在研究价值。
Objective To evaluate the immunoregulatory effects of the Lonicera water extract in the ovalbulmin （OVA）-sensitized BALB/c mice and to explore feasibility of treating food allergy with the traditional Chinese medicine （TCM）. Methods Forty female BALB/e mice aged 6 weeks fed with ovalbulmin-free feed, were randomly divided into 5 groups with 8 mice in each. Four groups were sensitized with OVA intraperitoneally two times and challenged intragastrieally four times. Groups H, M and L were treated respectively with high （ 100 mg/100 ml）, medium （50 mg/100ml） and low （25 mg/100 ml ） concentration of the Lonicera water extract at a dose of 0.3 ml/10 g body weight just 4 hours after the first challenge and then twice daily for 10 consecutive days. The mice in group Ch were used as positive control and were sensitized intraperitoneally and treated with normal saline solution intragastrieally daily. The mice in NS gruop were used as negtive control without sensitization and challenge. Just 1 hour after the last challenge, the mice in each group were sacrificed and specimens of jejunum were taken. Histological examinations on the jejunum specimens were performed after either HE or toluidine blue staining, the levels of histamine in gut of the mice were assayed with a fluorescent method ; the IFN-γ/ and IL-4 production in peripheral lymph node mononuelear cell （PLNMC） and the OVA-specific IgE levels in serum were measured by using ELISA;the mRNA expression of IL-12p40 in PLNMC of the mice was evaluated by RT-PCR; the footpad swelling reactions were assessed for the OVA-induced delayed hypersensitivity. Results （1）The inflammatory reactions were significantly inhibited in the mice of group H and M; the accumulated and degranulated mast cells in lamina propria were significantly reduced in the mice by gavage with 100% or 50% of the Lonicera extract, concomitant with the increased percentage of the intact mast cells. （2） The release of histamine in gut in the mice of group H and M was significantly reduced. （ 3 ） Either the IL-4 production and the ratio of IL-4/IFN-γ, in PLNMC or the IFN-γ, generation was significantly reduced in group H and M. （4）IL-12p40 mRNA expression in PLNMC was significantly reduced in group H and M. （5）The levels of OVA-specific IgE in serum were reduced in the mice of group H and M. （6） The footpad swelling reactions induced in the allergic mice were significantly inhibited after giving the Lonicera extract of the three different concentrations. Conclusion The Lonicera extract showed significant immunoregulatory effects in OVA-induced allergic mice model in this study. Lonicera extract may be of potential research value in treatment of both IgE and none IgE mediated food allergy.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2005年第11期852-857,共6页
Chinese Journal of Pediatrics
基金
重庆市卫生局中医药科研计划资助项目(渝中医2002-42)
