摘要
目的观察大鼠哮喘模型肺组织糖皮质激素受体（ＧＲ）和β－肾上腺素能受体（β－ＡＲ）水平及平喘药物对其影响。方法用放射性配基竞争结合法测定连续激发哮喘和药物治疗后各时点肺组织ＧＲ和β－ＡＲ。结果激发后第１天肺组织胞浆ＧＲ最大结合容量（Ｂｍａｘ）显著升高，３天后迅速降至低于正常水平，平衡解离常数（ｋＤ）值也显著降低。肺组织胞膜β－ＡＲ、Ｂｍａｘ和ｋＤ值激发后逐日下降，第１４天明显低于对照组。地塞米松可下调ＧＲ，雾化吸入作用弱于腹腔注射，而显著上调β－ＡＲ，雾化吸入者更明显。美喘清和舒喘灵治疗可防止ＧＲ下调。结论肺组织ＧＲ和β－ＡＲ随哮喘发作而变化，糖皮质激素（ＧＣ）可下调ＧＲ和上调β－ＡＲ，吸入优于全身用药。
ObjectiveToobservethechangesofglucocorticoidreceptor(GR)andbeta-adrenoreceptor(β-AR)oflungtissueandtheefectsoforalprocaterol,salbutamolandintraperitoneal(i.p.)orinhaled(i.h.)dexamethasone(Dex).MethodTheGRorβ-ARweredeterminedbyusing3H-dexamethasoneand3H-Dihydroalprenololasligandrespectively,ingroupsofratasthmamodelsat1,3,7,14thdayandgroupsreceivedantiasthmaticsonthe7thdayafterdailyconsecutivechalenges.ResultTheGRBmaxofasthmagroupsincreasedsignificantlyafterthefirstchalenge,butdecreasedsincethethirddaytoalowerlevelthanthatofhealthycontrol.TheKDalsodecreasedsig-nificantlysincethethirdday.Theβ-ARBmaxofasthmagroupgradualydecreasedtoasignificantlowlevelonthe14thday.TheDexi.p.grouphadthelowestGRBmaxandtheDexi.h.grouphadthehighestβ-ARBmaxa-mongthetreated.Intheprocaterolandsalbutamolgroup,therehadnodecreaseofGRBmaxcomparedwiththatofcontrols.ConclusionsTheGRandβ-ARlevelsoflungtissueschangedafterdailychalenges.TheGCplayedadown-regulationroleonGRandanup-regulationroleonβ-ARinlungtissues.TheDexi.h.hadmoreadvantagesthanDexi.p.intermsofGRandβ-ARregulations.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1996年第2期94-97,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
全军"八五"重点科研基金
关键词
哮喘
肺组织
受体
糖皮质激素
Β受体
平喘药
AsthmaDiseasemodel
AnimalReceptors
glucocorticoidReceptors
a-drenergic
