摘要
蝎毒组分Ⅲ（ｓｃｏｒｐｉｏｎｖｅｎｏｍｃｏｍｐｏｏｃｎｔⅢ，ＳＶＥⅢ）是从马氏钳蝎粗毒分离纯化获得的有效成分之一。本文用ＳＶＣⅢ处理体外培养的人喉癌ＨＥｐ￣（－２）细胞系和人直肠腺癌Ｃ１１８４细胞系，研究其抗癌活性。结果表明，以丝裂霉素做为阳性对照，ＳＶＣⅢ（１．６～８．０μｇ／ｍｌ）对两种癌细胞呈毒性和杀伤作用（Ｐ＜０．０１）。当ＳＶＣⅢ浓度为４．８、６．４和８．０μｇ／ｍｌ时，ＨＥｐ￣（－２）细胞和Ｃ１１８４细胞的克隆形成率降低（Ｐ＜０．０１），具有剂量依赖性，半数抑制浓度分别为５．０１μｇ／ｍｌ和６．６μｇ／ｍｌ。较大剂量ＳＶＣⅢ处理癌细胞２４ｈ，生长抑制率呈明显增高，以后趋于坪式。ＳＶＣⅢ还使有丝分裂指数和多核率减少。分化程度较高的ＨＥｐ－２细胞对ＳＶＣⅢ更为敏感。提示ＳＶＣⅢ是存在于蝎毒中的天然、高效低毒的抗癌成分。
Scorpion venom componentⅢ (SVC Ⅲ) was an effective component isolated andpurified from the scorpion (Buthus Martensii Karsch) venom crude (SVC). The antineoplastic ef-fect of SVC Ⅲ against in vitro cultured HEp￣(-2) human laryngeal cancinoma cell and C1184rectal adenocarcinoma cell was studied. The result showed that SVC Ⅲ at a coneentititionranging from 1.6μg/ml to 8.0μg/ml possessed the cytotoxicity and killing effect on both celllines(P<0.01). When the dosages of SVC Ⅲ were 4.8μg/ml, 6.4μg/ml and 8.0μg /ml, theircolony formation rates were lower than the blank control(P<0.01),which showed the halfinhibition effect of SVC Ⅲ in a dose-dependent manner. The half inhibition concerntrations(IC(5c))onHEp￣(-2) cell and C1184 cell were 5.e1μg/ml and 6.6lμg/ml, respanvely. The cell growth inhibitionrate was obviously increased when both cell lines were exposed to a higher dosage of SVC Ⅲfor24 hours. If cancinonia cells werecontinually treated with SVC Ⅲ, its inhibition trended to a pla-teau gradually. The reduction of the mitotic index and the mult-neclearrate was also induced bySVCⅢ. HEp￣(-2) cell, a highly differentiated cancinoma cell, wasevenmore sensitive to SVC Ⅲ .These results indicated that SVC Ⅲ may be a natural antineoplastic agent with higher effect and lower toxicity than SVC.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1996年第1期72-77,共6页
Chinese Journal of Pathophysiology
关键词
蝎毒类
细胞株
抗肿瘤药
马氏钳蝎
Scorpion venoms· Cell line· Antineoplastic agents
