摘要
目的探讨表皮生长因子(EGF)、表皮生长因子受体(EGFR)及两种原癌基因c-fos、c-jun,在口腔扁平苔藓中的基因表达、蛋白含量变化及其生物学意义。方法选择新鲜组织标本31例,包括糜烂型扁平苔藓10例、网状型扁平苔藓12例和正常口腔黏膜9例,使用逆转录聚合酶链反应技术(RT-PCR)检测EGF、EGFR、c-fos和c-jun基因在不同组织中的表达;同时使用免疫组化方法比较这4种蛋白在不同组织中的定位和表达量的变化。结果糜烂型扁平苔藓EGFR基因的mRNA和蛋白含量分别为(55.9±23.1)%、(71.1±10.1)%,均明显高于网状型扁平苔藓及正常口腔黏膜组织(P<0.05)。糜烂型扁平苔藓中的c-fos和c-jun基因表达呈相同的变化规律,两种基因的mRNA含量均显著高于正常口腔黏膜。糜烂型扁平苔藓的C-fos、C-jun蛋白的阳性细胞率分别是正常黏膜的1.3和1.5倍,蛋白含量明显高于正常口腔黏膜(P<0.01)。结论糜烂型扁平苔藓的癌变率可能大于网状型扁平苔藓,其中EGF及其受体引导的信号传导通路可能起着十分重要的作用。
Objective To explore the expression of epidermal growth factor （EGF）, epidermal growth factor receptor （EGFR） , c-fos and c-jun in oral lichen planus （OLP）. Methods The levels of gene expression of EGF, EGFR, c-fos and c-jun were detected with reverse transcription-polymerase chain reaction（RT-PCR） in 10 cases of erosive OLP, 12 cases of recticular OLP and 9 cases of normal oral mucosa. Protein contents and distribution of EGF, EGFR, C-fos and C-jun were examined with immunohistochemical technique in different tissues, Results The endogenous levels of EGFR mRNA and protein in erosive OLP were （55.9±23.1 ）% and （71.1±10.1 ）% , respectively, and significantly higher than those in recticular OLP and normal oral mucosa. The mRNA contents of c-fos and c-jun were significantly higher in erosive OLP than in normal oral mucosa. The positive cell rates of c-fos and c-jun protein in erosive OLP were 1.3 and 1.5 times of those in normal oral mucosa, respectively （ P〈0.05 ）. Conclusions The probability of carcinomatous change of erosive OLP might be higher than that of recticular OLP, in which EGF and EGFR might play an important role.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2005年第6期455-458,共4页
Chinese Journal of Stomatology
关键词
扁平苔藓
口腔
表皮生长因子
受体
表皮生长因子
原癌基因
Lichen planus, oral
Epidermal growth factor
Receptor,epidermal growth factor
Proto-oncogenes
