摘要
目的:观察三七通舒胶囊(主要成分是三七,成都华神集团股份有限公司生产,批号:940316)对大鼠局灶脑缺血再灌注性损伤后的神经保护作用。方法:实验于2003-02／2004-04在首都医科大学附属天坛医院神经外科试验中心完成。采用Koizumi法制备大鼠脑缺血再灌注模型,88只大鼠随机分3组:假手术组(8只);缺血再灌注组,缺血2 h后进行再灌注,依照再灌注后取样时间点的不同分为1,3．7,10,15 d共5组(每组 8只);三七通舒胶囊药物干预组,缺血2 h再灌注后2 h即开始给药, 依照再灌注后取样时间点的不同亦分为1,3,7,10,15 d共5组(每组8 只)。各时相点取材,苏木素-伊红染色观察病理改变,并进行脑含水量测定;免疫组化观察脑组织血管内皮生长因子(vascular endothelial growth factor,VEGF)及层粘连蛋白的表达变化,并使用多媒体彩色病理图像分析系统进行定量分析;利用草酸-焦锑酸钾电镜细胞化学技术观察缺血脑组织的超微结构改变。结果:脑缺血再灌注组与药物干预组脑水含量明显高于假手术组[以缺血再灌注7 d为例,缺血再灌注组、药物干预组、假手术组分别为 (70．79±2．44)％,(68.11±2．78)％,(65．76±2．57)％,P<0．05]。假手术组大鼠脑组织仅见少量VEGF表达,而层粘连蛋白表达较多[VEGF为 (18.37±4．86)个／高倍视野,层粘连蛋白为(62．76±5．99)个／高倍视野]。缺血再灌注组中,随着再灌注时间的延长,梗死灶周边区VEGF呈逐渐增强的趋势[再灌注1,3,10 d分别为(35.34±6．64),(56．96±5．86), (113．86±9．05)个／高倍视野],而层粘连蛋白的表达呈先减弱后逐渐增强的趋势[再灌注1,3,10 d分别为(30．86±4．94),(56．74±4．16),(88．36 ±3．03)个／高倍视野]。药物干预组中,随给药持续时间的延长,二者表达趋势同缺血再灌注组,且三七通舒胶囊药物干预组大鼠其VEGF、层粘连蛋白的表达较缺血再灌注组更强。结论:三七通舒胶囊可能减少缺血再灌注后脑含水量并增强VEGF及层粘连蛋白的表达,减轻缺血再灌注性脑损伤,从而起到了一定的神经保护及促进神经组织修复的作用。
AIM：To observe the neural protective effects of sanchi Tongshu capsule （composed of sanchi mainly, produced by CHENGDU HOIST INC.,LTD. with the batch number of 940316） after focal cerebral ischemia/reperfusion METHODS：The experiment was conducted at the Department of Neurosurgery, Affiliated Tiantan Hospital, Capital University of Medical Sciences between February 2003 and April 2004. Ischemia/reperfusion rat models were made with Koizumi method. Eighty-eight rats were assigned randomly into 3 groups： sham operation group （8 rats）, ischmia/reperfusion group, reperfusion was conducted at 2 hours after ischemia. According to the different sampling time after reperfusion, there were 1, 3, 7, 10 and 15 days, totally 5 groups （8 rats in each group）. Intervention group, after ischemia for 2 hours and reperfusion for 2 hours, drug was given. According to the different sampling time after reperfusion, there were also 1, 3, 7, 10 and 15 days, totally 5 groups （8 rats in each group）. Materials were drawn at every phase point. Pathological changes were observed with hematoxylineosin staining, and content of water in brain was detected; Vascular endothelial growth factor （VEGF） in brain tissue and expression of laminin were observed with immunohistochemical method. Quantitative analysis was performed with media mix color pathological image analysis system. Changes of uhrastructure in ischemia brain tissue were observed with oxalicpotassium pyroantimonate electron microscope cytoehemistry technique. RESULTS： Level of content of brain water in ischemia/reperfusion group and intervention group was obviously higher than that in sham operation group [taking ischemia/reperfusion for 7 days as an example, it was （70.79±2.44）%, （68.11±2.78）%, （65.76±2.57）% ,P〈0.05 in the ischemia/reperfusion group, intervention group and sham operation group, respectively]. There. were only a little VEGF expression in the sham operation group, while many linimins [VEGF was （18.37±4.86） per high power field; laminin was （62.76±5.99） per high power field]. In the ischemia/reperfusion group, with the prolongation of reperfusion time, VEGF around infarcted focus showed increasing trend lit was （35.34±6.64）, （56.96±5.86）, （113.86±9.05） pethigh power field, respectively at 1, 3 and 10 days of reperfusion], while the expression of laminin weakened, and then strengthened gradually [it was （30.86±4.94）, （56.74±4.16）, （88.36±3.03） per high power field, respectively at 1, 3 and 10 days of reperfusion]. In the intervention group, with the prolongation of administration time, the expressions of the two were the same to that in the isehemia/reperfusion group, and the expressions of VEGF and laminin in the intervention group were stronger than those in the isehemia/reperfusion group. CONCLUSION： The sanchi Tongshu capsule probably reduce the brain water, enhance expressions of VEGF and laminin and relieve brain damage after ischemia/reperfusion. Thus, it has the effects of certain neural protection and accelerating repair of repair if nervous tissue.
出处
《中国临床康复》
CSCD
北大核心
2005年第41期99-101,共3页
Chinese Journal of Clinical Rehabilitation
