摘要
目的探讨p53基因过表达介导不同转移潜能肿瘤细胞的凋亡分子机理,寻找与细胞凋亡和肿瘤转移的相关基因。方法应用mRNA差异显示方法分析腺病毒介导的野生型p53基因感染不同转移潜能的肺癌细胞系前后存在的表达差异基因,并用逆转录-聚合酶链反应、Northern印迹和Western印迹方法加以验证。结果分析发现,在p53基因诱导后ANNEXIN A2基因的表达显著差异有统计学意义,经p53基因诱导后ANNEXINA2基因的表达有明显下调。并且以Anip973细胞的表达缺失最为显著。经逆转录-聚合酶链反应、Northern印迹和Western印迹方法也验证了这种差异的存在。结论表明ANNEXINA2基因的表达与p53基因诱导的肿瘤细胞凋亡存在密切的相关性。
Objective To identify the relationship between p53-dependent apoptosis associated genes and tumor metastasis. Methods mRNA differential display （mRNA DD） was adopted for gene cloning after the different metastatic potential lung cancer cell lines were infected by Advp53 （a reconstructed adenovirus encoding wild typep53 gene）. RTPCR, Northern blot and Western blot assays were used to confirm the result from mRNA DD, Results After induction by p53 gene, the ANNEXIN A2 gene had differential expression in the ceil lines; its level was down-regulated in all the cells infected by Advp53 gene, especially in the Anip973 ceil lines with high metastatic potential. RT-PCR, Northern blot and Western blot assays confirmed the consequence. Conclusion The experimental data suggest that the ANNEXIN A2 gene may relate to cellular apoptosis induced by p53 gene. The affirmative relationship between ANNEXIN A2 gene and p53 needs further investigation.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2005年第6期661-664,共4页
Chinese Journal of Medical Genetics
基金
国家自然科学基金(30471949)
高等学校优秀青年教师教学科研奖励计划
黑龙江省科学技术攻关项目(GB03C601-1)
黑龙江省卫生厅资助项目(2003-103)
国家高技术研究发展计划(2002BA711)~~
