摘要
从膨润土中分离出一株对酸性蓝B具有降解效果的镰刀菌(Fusariumsp.)HJ01,研究了其生长特征和动力学模型模拟、降解酶的性质以及对酸性蓝B的脱色效果.结果表明:菌株生长的适宜条件为:pH范围为6~7,蔗糖为碳源,NH4Cl为氮源.菌体生长符合改进的logistic law模型.SDS-PAGE分析HJ01菌株所产漆酶的相对分子量约为66×103,在低氮条件下漆酶活性高达431~812U·mg^(-1)·s^(-1).菌体培养96h后加入到含100mg·L^(-1)酸性蓝B的培养基中,再培养96h,酸性蓝B的脱色率达100%.紫外光谱分析表明,酸性蓝B的发色基团蒽醌环被漆酶破坏.
An acid blue B-degrading strain （ Fusaritun sp. ）HJ01 was isolated from bentonite. The growing characteristics and kinetics, the characteristics of enzymatic biodegradafion of Fusarium sp. HJ01 were analyzed. And the effects of biomass on decolorization of acid blue B in aqueous samples were reported. The results showed that the pH range between 6 and 7 for growth of Fusarium sp. HJ01 was feasible, and the sucrose and NH4Cl, is also feasible for carbon source and nitrogen source respectively. The growth kinetics of Fusarium sp. HJ01 was fitted by the modified logistic law model. The molecular weight of laccase from Fusarium sp. HJ01 was approximately 66kD analyzed by SDS-PAGE, and laccase activity was up to from 431 U· mg^- 1 . s ^- 1 to 812 U· mg^-1 · s^-1 at the condition of low nitrogen source. The Fusarium sp. HJ01 incubated for 96 h at 25 ℃ were added to the liquid medium containing acid blue B （ 100 mg· L^-1 ）. And when cultured continuously for another 96 h, the decolorization rate of acid blue B was up to 100%. The ultraviolet-visible spectrum showed that the chromophore anthraquinone ring of acid blue B was broken down by laccase.
出处
《环境科学学报》
CAS
CSCD
北大核心
2005年第12期1641-1646,共6页
Acta Scientiae Circumstantiae
关键词
镰刀菌
脱色
酸性蓝B
Fusarium
decolorazition
Acid blue B
