摘要
目的 为在福州地区建立原发无精子症、严重少精子症Y染色体AZF区域微缺失分子诊断方法。方法 根据Y 染色体长臂无精子因子区(AZF)序列标签位点(sequence taggedsites,STS)和短臂SRY基因等共设计10对引物,采用多重聚合酶 链反应-琼脂糖凝胶电泳方法对95例原发无精子症、42例少精子症、20例精子活动力>50％男性患者及50例正常生育男性 外周血DNA进行Y染色体AZF区域微缺失分析。结果95例原发性无精子症患者检出13例AZF区域STS位点缺失,占13．7％,42 例严重少精子症患者检出AZF区域STS位点缺失5例,占11．9％,正常生育男性、精子活动力>50％患者无一例AZF微缺失。 结论 Y染色体AZF区域微缺失与男性无精子症、严重少精子症密切相关。
Objective： To establishe gene diagnosis assey of Y- chromosome AZF microdeletion for male azoospermia or oligozoospermia in Fuzhou. Methods： Using 10 Tag Sequence Site（SIS） primers binding method and multiplex PCR with agarose gel elec- trophoresis were performed to detect AZF microdletions in 95 patients azoospermia and 42 patients with oligozoospermia .Results： 13 AZF microdeletion were conformed 95 patients azoospermia （13.7%） and 5 with 42 patients with oligozoospermia （11.9%）. No AZF microdeletion was form in 50 patients normal male and 20 patients male where active rate of sperm were less then 50%. Conclusions： Y - chromosome AZF microdeletion was associated with male azoospermia or oligozoospermia.
出处
《中国优生与遗传杂志》
2005年第11期39-41,共3页
Chinese Journal of Birth Health & Heredity
