摘要
从新疆克拉玛依地区皮肤利什曼病患者皮损内分离的３株婴儿利什曼原虫，接种至草原兔尾鼠或背纹仓鼠的腹腔／睾丸内后，引起内脏感染。鼠的病理变化与从内脏利什曼病人体内分离出来的婴儿利什曼原虫／杜氏利什曼原虫引起的一致。ＢＡＬＢ／ｃ小鼠皮下接种原虫后，既不发生皮肤损害，也不引起内脏感染。猴的皮下注射该原虫后，仅产生含虫的皮肤损害而不引起内脏感染。皮肤利什曼病人的皮损组织置ＮＮＮ培养基内培养时，在培养基内难以查获前鞭毛体，用上述感染鼠的脾组织置该培养基内培养，可以使前鞭体生长繁殖。无鞭毛体的超微结构显示，膜下微管数为７４±７，周长为８．８６±１．２５μｍ，原虫的大小指数为６．６１±１．５６。通过本文结果及文献复习，作者指出：中国的婴儿利什曼原虫是由基因型存在一定差异和表型呈多态性的不同品系组成的复合体。
It is difficult to isolate Leishmania by using the NNN medium direct cultivation of ground tis-sue taken from lesions of cutaneous leishmaniasis(CL).When Cricetulus barabensis and Lagurus lagurus wereinoculated intraperitoneally or intratesticularly with suspension of CL skin lesions,systematic infection devel-oped,involving spleen,liver and bone marrow which contained numerous parasites. The pathological changesin these organs are similar to those in C. barabensis and L. lagurus inoculated with Leishmania donovani or L.infantum. When infected spleen tissue of the laboratory animals were put into NNN medium,proliferation ofpromastigotes was viewed.Leishmania isolated from 2 cases were injected subcutaneously into face of 2 mon-keys,ulceration containing Leishmania was observed at the site of inoculation and no visceral involvement.BALB/c mice were insusceptible to the infection.Ultrastructural studies revealed that the amastigotes of theisolated of Leishmania were 3.68±0.6μmin length and 1.80±0.28 μm in width,the size index being 6.61±1.56 and the mean number of subpellicular microtubules in the section at nuclear level was 74±7.DNA hy-bridization of Leishmania stocks isolated from CL cases in 1994 and 1995 revealed their homology to L. in-fantum. Based on the results of this paper and other work relative to L. infantum studies in China,the authorsuggested that L. infantum sl in China may be a complex which is constituted of some different strains.
出处
《地方病通报》
1996年第3期1-5,共5页
Endemic Diseases Bulletin
基金
国家自然科学基金
关键词
皮肤利什曼病
病原生物学
婴儿
Cutaneous leishmaniasis
Parasite biology
Leishmania infantum
Karamay,Xinjiang,China
