摘要
目的观察柯里拉京(Corilagin)对人喉癌细胞系Hep-2的杀伤作用。方法在体外细胞培养的基础上,通过倒置显微镜、MTT比色法、流式细胞仪观察Corilagin作用后Hep-2细胞的形态学、增殖率和细胞周期的改变。结果Corilagin作用后的Hep-2细胞形态发生了明显变化;其抑制增殖作用呈量效、时效关系;Corilagin可阻肿瘤滞细胞于S期,并可诱导细胞凋亡。结论Corilagin能显著抑制Hep-2细胞增殖,可能与阻滞细胞于S期和诱导凋亡有关,Corilagin抗肿瘤作用值得进一步研究。
Objective To study the Cytotoxic effect of Corilagin on human laryngeal carcinoma Hep-2 cell in vitro. Methods Cell morphology, MTT assay and flow cytometry were used to examine the Cytotoxic effect. Results Cells treated with Corilagin had much changes in morphology. The growth inhibition effect of Corilagin on Hep-2 cells were dosage and time dependent. The result of flow of cytometry showed cells were blocked in S phase and were induced to apoptosis. Conclusions Corilagin can inhibite the growth of Hep-2 cells effectively, the effect maybe have relation to the S phase arrest and the induction of apoptosis.
出处
《锦州医学院学报》
2005年第4期22-24,共3页
Journal of Jinzhou Medical College
关键词
柯里拉京
喉癌
凋亡
细胞周期
Corilagin
laryngeal carcinoma
apoptosis
cell cycle
