摘要
目的:探讨白细胞介素-6(IL-6)反义寡核苷酸脂质体预防后发性白内障的可行性和有效性。方法:30只健康新西兰大白兔随机分为三组,每组10只,所有白兔左眼均行透明晶状体囊外摘除术。反义组术眼前房注射IL-6反义寡核苷酸脂质体;单纯组术眼前房注射单纯脂质体;对照组不注射任何药物。分别于术前1天,术后1、3、7、14和30天抽取房水,用ELISA双抗体免疫夹心法检测房水中IL-6的含量,定期裂隙灯显微镜及检眼镜观察眼前节及眼底变化情况,术后3个月行数码裂隙灯后囊照相和组织病理学检查。结果:①术后3个月反义组、单纯组及对照组分别有7、10及10只眼发生晶状体后囊膜混浊,差异有显著性意义(P=0.037)。②术后反义组的IL-6平均浓度低于单纯组及对照组,其差异有显著性意义(P<0.05),单纯组与对照组间无显著性差异。③临床观察和组织病理学检查未发现眼内毒性反应。结论:IL-6反义寡核苷酸脂质体对后发性白内障可能有一定的抑制作用。
Objective：To investigate the safety and efficacy of interleukin-6 antisense oligonucleotide liposomes on the experimental animals for prevention of after cataract. Methods：Thirty healthy NewZealand rabbits（30 left eyes） received extracapsular lens extraction, and were randomly divided into 3 groups： anterior chamber injection of 0. 1 ml of interleukin-6 antisense oligonucleotide liposomes （ Group A ）, blank liposomes （ Group B ） after operation respectively ; and control group （ Group C ）. All left eyes were examined by slit-lamp microscope and ophthalmoscope regularly. The aqueous humor IL-6 concentrations were measured 1 day before operation, and 1 day, 3 days, 7 days, 14 days and 30 days after operation through enzyme-linked immunoabsorbent assay. Three months later, photographs of the posterior lens capsules were taken through digital slit-lamp microscope and the histopathological changes of posterior lens capsules were observed by light microscope. Results：①Three months after the operation, the number of eyes developing posterior capsule opacification in Group A, B and C were 7, 10 and 10 respectively, with significant difference （P=0.037）.② The average value of Group A was lower than Group B and C, with statistically significant difference（P〈0.05 ）. ③The coniunctiva, cornea, iris. cil-iary body and retina remained intact, without obviously toxic reaction. Conclusion： The injection of interleukin-6 antisense oligonucleotide liposomes into the anterior chamber may prevent after cataract to some extent.
出处
《医学研究生学报》
CAS
2005年第12期1083-1086,i0018,共5页
Journal of Medical Postgraduates
基金
湖北省卫生厅科研基金资助项目(批准号:301140324)
