摘要
目的建立615小鼠结肠癌肝转移模型.方法将1×106个/ml小鼠结肠癌细胞(CT26)悬液0.1ml接种于615小鼠脾被膜下,分别于术后第1周、第2周、第3周、第4周处死2～3只小鼠,观察腹腔内肿瘤生长情况,其余小鼠待其自然死亡,观察生存天数.结果肝转移发生率为93.33%,小鼠平均荷瘤生存时间为(28.60±7.63)d.病理结果提示肝转移瘤细胞与脾脏肿瘤细胞结构相似,符合低分化腺癌的特征.结论本研究成功建立了小鼠结肠癌肝转移模型,为筛选抗肿瘤药物和研究肿瘤转移行为提供了有用的模型,具有一定的应用价值.
Objective To establish a colonic carcinoma liver metastases model for experimental studies in 615 mice. Methods Liver metastases were established in 615 mice by intrasplenic injection of colonic carcinoma cells（CT26） with 1×10^6 cells in 0.1 ml solution. After surgery 1、2、3、4 week, 2～3 mice were sacrificed every week, and the others were used to observe the survival time. The liver metastases conditions were dissected and recorded for all animals. Results The incidence rates of liver metastases was 93.33% , the average survival time of tumor bearing animals was （28.60±7.63） days. The pathological results showed that tumor cells of liver metastases accorded with poorly differentiated adenocarcinoma. Conclusion This is a useful model to select antineoplastic and to study metastatic behaviors.
出处
《医药论坛杂志》
2005年第23期26-27,共2页
Journal of Medical Forum
