摘要
以鹅副粘病毒HZ株的基因组RNA为模板,采用RT-PCR技术扩增出F基因片段,然后将其克隆到pUCm-T载体中。经转化、筛选、酶切和PCR鉴定后,初步获得含鹅副粘病毒F基因的阳性克隆,并进行序列测定验证。结果表明,该基因由1 662个核苷酸组成,共编码553个氨基酸。与GenBank下载的9株参考毒株的F基因作同源性比较,发现HZ株与ZJ1、F48E9、Lasota等毒株的核苷酸同源性分别为98.3%、85.4%、82.7%;氨基酸同源性分别为97.8%、90.6%、88.1%。说明HZ株与经典的鸡新城疫病毒(NDV)在F基因上存在很大的差异,而与近年来的分离株亲缘关系较近。
The genomic RNA from purified GPV-HZ strain was extracted and used as a template for PCR. The fragment of the fusion gene was amplified by RT-PCR with the primers designed according to the sequence of NDV from Genbank and cloned into pUCm-T and sequenced. As a result, the ORF of fusion gene was 1662 bp in length, encoding 553 amino acid. By sequence comparison among HZ and ZJ1, F48E9 and Lasota strains, homologies of nucleotide sequence were 98.3% , 85.4% and 82.7% respectively. Homologies of the amino acid were 97.8% , 90. 6% and 82. 7% respectively. The results indicated that the mutation on the F gene had varied largely, compared with classic NDV.
出处
《畜牧与兽医》
北大核心
2005年第9期6-8,共3页
Animal Husbandry & Veterinary Medicine
基金
浙江省自然科学基金(YA30446)
关键词
鹅副粘病毒
F蛋白基因
序列分析
goose＇s paramyxovirus
fusion gene
sequence analysis
