摘要
[目的]探讨核转录因子-kappaB(NF-κB)在PM2.5染毒小鼠急性肺损伤过程中的作用。[方法]采集上海市交通区PM2.5,设低(1.6mg/kg)、中(8.0mg/kg)、高(40mg/kg)浓度组和对照组,通过气管滴注染毒C57BL/6小鼠24h后,用RT-PCR方法对肺组织中肿瘤坏死因子α(TNF-α)、白细胞介素1(IL-1)、白细胞介素6(IL-6)、一氧化氮合酶(NOS)和NF-κB的基因表达水平进行检测,观察PM2.5染毒后各指标基因表达的改变情况。[结果]小鼠肺组织中TNF-α、IL-1、IL-6和NOS基因表达水平各染毒组均显著高于对照组(P<0.01),并随着染毒浓度的增加而增加,NF-κB的基因表达水平与上述指标基因水平显著相关,相关系数均高于0.9(P<0.01)。[结论]PM2.5能够引起小鼠肺组织中细胞因子和氧化酶基因水平表达增加,从而造成肺组织的免疫和氧化损伤,NF-κB在PM2.5引起急性肺损伤过程中可能起到重要的调节作用。
[ Objective ] To explore the role of NF-κB in the course of acute lung injury induced by PM2.5 in mice. [ Methods ] C57BL/6 mice which are sensitive to PM were randomly divided into four groups. The exposed groups （ low, middle and high ） were instillated with suspension of PM2.5, and the control group was instillated saline via trachea. 24 hours after instillation mRNA expressions of TNF-α , IL-1, IL-6, NOS and NF-κB in lung were examined with RT-PCR. [ Results ] mRNA expressions of TNF-α , IL-1, IL-6, NOS in the instillated groups were higher than those in the control group（ P 〈 0.01 ）, and increased with the dose. Gene expression of NF-κB was related to these genes expressions and the correlation coefficients were more than 0.9（ P 〈 0.01 ）. [ Conclusion ] PM2.5 exposure could increase gene expression of cytokines and oxidase of lung, which responsible for immune injury and oxidative injury. NF-κB may have a key role in the course of lung injury induced by PM2.5.
出处
《环境与职业医学》
CAS
北大核心
2005年第6期483-485,501,共4页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金资助项目(编号:30271106)
国家自然科学基金重点课题项目(编号:10490182)
