摘要
目的建立一种稳定、简便的昆布多糖含量测定方法。方法采用精制昆布多糖测得昆布多糖对葡萄糖的换算因子后,样品经除杂处理后,用苯酚-硫酸法测定昆布中多糖的含量。结果用此方法测定多糖的标准曲线,其相关系数为0.9998,平均回收率为97.35%,重现性相对标准偏差为4.68%。结论本方法所需仪器简单、操作方便,适合于昆布及其制品的多糖含量的常规检测。
Objective To develop a stable and simple method to measure the polysaccharide in Ecklonia kurome Okam. Methods After the conversion coefficient of polysaccharide from Ecklonia kurome Okam to glucose was obtained, the content of polysaccharide was measured by phenol-sulfuric acid method. Results The correlation coefficient of standard curve was 0. 9998, the recovery rate was 97.35% and RSD was 4.68%. Conclusion The method was convenient for content determination of polysaccharide from Ecklonla kurorne Okam as well as its related products.
出处
《中国海洋药物》
CAS
CSCD
2005年第6期47-48,共2页
Chinese Journal of Marine Drugs
关键词
黑昆布
多糖
含量测定
Ecklonia kurome Okam
polysaccharide
determination
