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DDT降解菌株DB-1的分离、系统发育及降解特性 被引量:13

Isolation, phylogenesis and degradation characters of DDT-degrading bacterium strain DB-1.
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摘要 从农药厂下水道污泥中分离、筛选到1株能够在好氧条件下降解DDT的细菌菌株DB-1,根据表型特征、生理生化特性及16S rDNA序列的系统发育分析,将菌株DB-1初步鉴定为鞘氨醇单胞菌属(Sphingomonas sp).该菌株能在含酵母膏(40mg/L)的DDT(40mg/L)无机盐液体培养基中降解DDT,10d降解率达到83.6%,菌株DB-1在25~30℃长势较好,最适生长pH值为8.0. A bacterium strain DB- 1 capable of degrading DDT under the aerobic condition was isolated and screened from the sewer sludge of a pestcide factory. Based on the phenotype, physiological and biochemical characteristics, and the phylogenetic analysis of 16S rDNA sequence, the strain DB-1 was identified preliminarily as Sphingomonas sp. This strain could degrade DDT in inorganic salt liquor culture base of DDT (40mg/L) containing yeast cream (40mg/L), with degradation efficiency reaching 83.6% in 10 days. The strain DB-1 growed better at 25-30℃, with most adaptable pH value of 8.0.
出处 《中国环境科学》 EI CAS CSCD 北大核心 2005年第6期674-677,共4页 China Environmental Science
基金 国家"863"项目(2004AA246070 2004AA214102) 国家自然科学基金资助项目(30400013) 江苏省科技厅项目(BG2005322)
关键词 DDT 农药残留 生物降解 系统发育分析 DDT pestcideresidue biodegradation phylogenetic analysis
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参考文献9

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