摘要
对安徽铜陵五公里尾矿区三叶草复垦后的土壤微生物活性和群落的功能多样性研究表明,复垦后每克土壤微生物细菌数已达2.3×107个,并存在一定量耐Cu2+、Cd2+、Zn2+、Cr6+、Pb2+、Ni2+的菌株,但种类少,主要为芽孢杆菌属、假单胞菌属和短杆菌属。BIOLOG测试结果显示,铜镉重金属离子的复合污染使土壤微生物群落丰富度降低;外源耐铜镉微生物菌剂的添加使尾矿区土壤微生物群落代谢剖面(AWCD)变化明显,提高微生物对单一碳源底物的利用能力,增加利用不同碳底物的微生物数量,减轻了重金属污染引起的土壤微生物群落功能多样性的下降,为尾矿区生境加速恢复提供一条有效途径。
Microbial activity and microbial community diversity were determined in polluted soils of copper main tailings which were cultivated by Trifoliumpratense in Tongling (Wugongli), Anhui province. Soil bacteria number reached 2.3×10^7 cfu/g soil. There were a lot of tolerant-bacteria to Cu^2+-, Cd^2+-, Zn^2+-, Cr^6+-, Pb^2+-, Ni^2+-tolerant in these soils. But the variety of these bacteria was few. They mainly belongs to Bacillus, Pseudomonas and Brevibacterium. BIOLOG tests showed that community richness decreased in mine soils after polluted copper and cadmium ions. It suggested that soil microbial metabolic profiles (AWCD) values changed significantly and utilization ability and quantities of microbial communities for different carbon substrates were increased by inoculating copper and cadmium tolerant-bacteria. The decline of soil microbial community functional diversities would be prevented in polluted soils. Our study provides an available approach to quickly resume mine-tailings areas.
出处
《生态环境》
CSCD
北大核心
2005年第6期876-879,共4页
Ecology and Environmnet
基金
安徽省02高校青年基金项目(143-140136)
关键词
铜尾矿
铜镉污染
耐铜镉菌剂
土壤微生物群落功能多样性
copper mine tailings
opper and cadmium contamination
opper and cadmium tolerant-bacteria
functional diversities of soil microbial communities