摘要
利用银杏单细胞克隆系研究银杏单细胞生长稳定性与细胞均一性之间的关系。结果显示:培养基过滤杀菌是促进细胞生长的好方法,当悬浮培养愈伤组织3代和向培养基中加入0.5g/L的酪蛋白水解物,能显著增加植板率,单细胞克隆的植板率为27.04%。克隆系GB-11平均生长率为0.55gfw/gfw·d。
The relationship between stability of ginkgo single-cells and cells uniformity its was studied by cells-cloning series of ginkgo. The results indicated： sterilization of medium by filtration was the good method to promote cells growth. When suspended culture of the 3rd generation callus, adding 0.5g/L hydrates of casein in medium might increase planting efficiency. The planting efficiency of single-cells cloned was 27.04%. The average growth rates of GB- 11 cloning lines reached 0.55g fw/g fw · d.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第12期68-70,共3页
Food Science
关键词
银杏
愈伤组织
单细胞克隆
植板率
生长速率
ginkgo
callus
single-cells clone
plating efficiency
growth rates
