摘要
分别用聚乙烯亚胺(PEI)和戊二醛(Glu)溶液在压电石英晶体银电极上固定梅毒抗体来检测不同浓度的标准抗原溶液.抗体在晶体表面固化后通过原子力显微镜(AFM)显示依然保持Y型分子结构.石英晶体的响应线性范围5×10-5～1.25×10-4 g/mL,相关系数为0.9976,最佳响应pH值为7.5.在检测时通过和BSA相对照,传感器有良好的选择性.最后,又对传感器重复性作了一定探讨.
We utilized polyethylenimine （PEI） and Glu solution to immobilize the antibody of Treponema Pallidum on the silver electrode of piezoelectric quartz crystal and detect the standard antigen solution at different concentration. The antibody keeps the intrinsic Y type molecular structure revealed by AFM. The resonant range of the sensor is between 5 × 10^-5～1. 25 × 10^-4g/mL and the correlation coefficient is 0. 9976, the optimal resonance pH is 7, 5. We found the sensor having good selectivity in comparison with the method using BSA. At last, a discussion on the reproducibility of the sensor is presented.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2005年第6期1215-1218,共4页
Journal of Biomedical Engineering
基金
广东省重点攻关项目资助(2km04301s)
全军医卫科研基金项目资助(98M038)
关键词
聚乙烯亚胺
戊二醛
梅毒
压电石英晶体
免疫传感器
Polyethylenimine （PEI） Glu Treponema pallidum Piezoelectric quartz crystal Immunosensor
